Co-culture of Human Stem Cell Derived Neurons and Oligodendrocyte Progenitor Cells

Crosstalk between neurons and oligodendrocytes is essential for correct mind functioning. Multiple co-culture strategies have been developed to check oligodendrocyte maturation, myelination or the impact of oligodendrocytes on neurons. However, most of these strategies comprise cells derived from animal fashions. In the present protocol, we co-culture human neurons with human oligodendrocytes. Neurons and oligodendrocyte precursor cells (OPCs) had been differentiated individually from pluripotent stem cells in keeping with beforehand revealed protocols. After the harvesting of cells from bone marrow by flushing the femoral diaphysis and enzymatic digestion of stomach and inguinal adipose tissues, MSCs are chosen by their adherence to the plastic tissue tradition dish.

To examine neuron-glia cross-talk, neurons and OPCs had been plated in co-culture mode in optimized situations for extra 28 days, and ready for OPC maturation and neuronal morphology evaluation. To our information, that is one of the primary neuron-OPC protocols containing all human cells. Specific neuronal abnormalities not noticed in mono-cultures of Tuberous Sclerosis Complex (TSC) neurons, turned obvious when TSC neurons had been co-cultured with TSC OPCs. These outcomes present that this co-culture system can be utilized to check human neuron-OPC interactive mechanisms concerned in well being and illness.

Since their discovery, mesenchymal stromal cells (MSCs) have obtained loads of consideration, primarily as a consequence of their self-renewal potential and multilineage differentiation capability. For these causes, MSCs are a great tool in cell biology and regenerative medication. In this text, we describe protocols to isolate MSCs from bone marrow (BM-MSCs) and adipose tissues (AT-MSCs), and strategies to tradition, characterize, and differentiate MSCs into osteoblasts, adipocytes, and chondrocytes. Within 7 days, MSCs attain 70% confluence and are prepared for use in subsequent experiments. The protocols described listed here are simple to carry out, cost-efficient, require minimal time, and yield a cell inhabitants wealthy in MSCs.

 

The choroid plexus consists of a community of secretory epithelial cells localized all through the lateral, third and fourth ventricles of the mind. Cerebrospinal fluid (CSF) is generated by the choroid plexus and launched into the ventricular atmosphere. This biofluid accommodates an enriched supply of proteins, ions, and different signaling molecules for extracellular assist of neurons and glial cells inside the central nervous system. Given that different cells within the mind additionally launch elements into the CSF, in vitro investigations of choroid plexus operate are essential to isolate processes selectively occurring inside and launched from this tissue.

Here, we describe a protocol to isolate choroid plexus tissue from every of the ventricular areas, and the cell tradition situations required to assist development and upkeep of these epithelial cells. This approach permits for investigations of the practical significance of the choroid plexus, akin to for the examination of stimuli selling the discharge of development elements and extracellular vesicles (e.g., exosomes and microvesicles) from ventricle-specific choroid plexus epithelial cells.

Investigations into glial biology have contributed considerably in understanding the physiology and pathology of the nervous system. However, intricacies of the neuron-glial and glial-glial interactions in vivo current vital challenges whereas delineating the person cell-type contributions, thus making the in vitro strategies exceedingly related to check glial biology. However, acquiring optimum yield together with excessive purity has been difficult for microglial cultures. Here we current a easy protocol to determine enriched astroglial in addition to microglial cultures from the neonatal rat spinal twine. This methodology ends in extremely enriched astroglial and microglial cultures with maximal yield.

 

Persistence of the human hepatitis B virus (HBV) requires the upkeep of covalently closed round (ccc)DNA, the episomal genome reservoir in nuclei of contaminated hepatocytes. cccDNA elimination is a serious goal in future healing therapies presently underneath improvement. In cell tradition primarily based in vitro research, each hybridization- and amplification-based assays are presently used for cccDNA quantification. Southern blot, the present gold customary, is time-consuming and not sensible for a big quantity of samples. PCR-based strategies present restricted specificity when extreme HBV replicative intermediates are current. We have not too long ago developed a real-time quantitative PCR protocol, by which complete mobile DNA plus all types of viral DNA are extracted by silica column.

Subsequent incubation with T5 exonuclease effectively removes mobile DNA and all non-cccDNA types of viral DNA whereas cccDNA stays intact and can reliably be quantified by PCR. This methodology has been used for measuring kinetics of cccDNA accumulation in a number of in vitro an infection fashions and the impact of antivirals on cccDNA. It allowed detection of cccDNA in non-human cells (major macaque and swine hepatocytes, and so forth.) reconstituted with the HBV receptor, human sodium taurocholate cotransporting polypeptide (NTCP). Here we current an in depth protocol of this methodology, together with a piece flowchart, schematic diagram and illustrations on find out how to calculate “cccDNA copies per (contaminated) cell”.

anti-Betacellulin
YF-PA10526	Abfrontier	50 ug	EUR 363
Description: Mouse polyclonal to Betacellulin

anti-Betacellulin
YF-PA10527	Abfrontier	100 ul	EUR 403
Description: Rabbit polyclonal to Betacellulin

anti-Betacellulin
YF-PA10528	Abfrontier	100 ug	EUR 403
Description: Rabbit polyclonal to Betacellulin

anti- Betacellulin antibody
FNab00883	FN Test	100µg	EUR 505.25
Recommended dilution: WB: 1:500-1:2000 Immunogen: betacellulin Uniprot ID: P35070 Gene ID: 685 Research Area: Cancer, Signal Transduction, Immunology
Description: Antibody raised against Betacellulin

Anti-Betacellulin antibody
PAab00883	Lifescience Market	100 ug	EUR 355

Betacellulin, Human
HY-P7005	MedChemExpress	10ug	EUR 211

Betacellulin, human
RC218-23	Bio Basic	5ug	EUR 104.38
Product category: Proteins/Recombinant Proteins/Growth Factors

Anti-Betacellulin/Btc Antibody
A02171-1	BosterBio	100ug/vial	EUR 334

Rabbit Polyclonal antibody Anti-CRBN
Anti-CRBN	ImmunoStep	50 µg	EUR 349

Recombinant Human Betacellulin
7-00223	CHI Scientific	5µg	Ask for price

Recombinant Human Betacellulin
7-00224	CHI Scientific	20µg	Ask for price

Recombinant Human Betacellulin
7-00225	CHI Scientific	1mg	Ask for price

Bovine Betacellulin
ARU020	GroPep	20 µg	EUR 253

Bovine Betacellulin
ARU100	GroPep	100 µg	EUR 346

Betacellulin, Mouse
HY-P7329	MedChemExpress	50ug	EUR 612

Betacellulin antibody
70R-BR013	Fitzgerald	50 ug	EUR 273
Description: Affinity purified Rabbit polyclonal Betacellulin antibody

Betacellulin protein
30R-AB028	Fitzgerald	20 ug	EUR 273
Description: Purified recombinant Human Betacellulin protein

Betacellulin (HEK293-expressed)， Human
HY-P7328	MedChemExpress	10ug	EUR 234

Human Betacellulin (bTC) Protein
20-abx065524	Abbexa	EUR 648.00 EUR 272.00 EUR 1943.00 EUR 759.00 EUR 467.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-12 working days.

Recombinant Human Betacellulin Protein
PROTP35070-2	BosterBio	20ug	EUR 317
Description: Betacellulin is an EGF-related polypeptide growth factor that signals through the EGF receptor. It is produced in several tissues, including the pancreas, small intestine, and in certain tumor cells. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. Human Betacellulin is initially synthesized as a glycosylated 32.0 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce the mature sequence. Recombinant human Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein.

Polyclonal Goat anti-GST α-form
GST-ANTI-1	Detroit R&D	50 uL	EUR 280

Polyclonal Goat anti-GST μ-form
GST-ANTI-2	Detroit R&D	50 uL	EUR 280

Polyclonal Goat anti-GST p-form
GST-ANTI-3	Detroit R&D	50 uL	EUR 280

Betacellulin (BTC) Antibody
20-abx111218	Abbexa	EUR 732.00 EUR 398.00	150 ul 50 ul
Shipped within 5-10 working days.

Betacellulin (bTC) Antibody
20-abx100739	Abbexa	EUR 342.00 EUR 133.00 EUR 982.00 EUR 495.00 EUR 286.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-12 working days.

Betacellulin (bTC) Antibody
20-abx100740	Abbexa	EUR 398.00 EUR 133.00 EUR 1107.00 EUR 537.00 EUR 314.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-7 working days.

Betacellulin (bTC) Antibody
20-abx175568	Abbexa	EUR 1135.00 EUR 551.00	1 mg 200 ug
Please enquire.

Betacellulin (bTC) Antibody
20-abx175569	Abbexa	EUR 1177.00 EUR 578.00	1 mg 200 ug
Please enquire.

Betacellulin (bTC) Antibody
20-abx175570	Abbexa	EUR 1177.00 EUR 578.00	1 mg 200 ug
Please enquire.

Betacellulin (bTC) Antibody
20-abx175571	Abbexa	EUR 1288.00 EUR 620.00	1 mg 200 ug
Please enquire.

Betacellulin (BTC) Antibody
abx230883-100ug	Abbexa	100 ug	EUR 481
Shipped within 5-12 working days.

Betacellulin (bTC) Antibody
20-abx171411	Abbexa	EUR 787.00 EUR 411.00	1 mg 200 ug
Please enquire.

Betacellulin, Murine Recombinant
7119-10	Biovision		EUR 196

Betacellulin, Murine Recombinant
7119-50	Biovision		EUR 675

Recombinant Bovine Betacellulin
7-00226	CHI Scientific	5µg	Ask for price

Recombinant Bovine Betacellulin
7-00227	CHI Scientific	20µg	Ask for price

Recombinant Bovine Betacellulin
7-00228	CHI Scientific	1mg	Ask for price

Recombinant Betacellulin (bTC)
4-RPA112Hu01	Cloud-Clone	EUR 458.40 EUR 226.00 EUR 1444.00 EUR 548.00 EUR 996.00 EUR 370.00 EUR 3460.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Uniprot ID: P35070 Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. Form: Freeze-dried powder Predicted Molecular Mass (KD): 10.2kDa Isoelectric Point: Inquire
Description: Recombinant Human Betacellulin expressed in: E.coli

Recombinant Betacellulin (bTC)
4-RPA112Mu01	Cloud-Clone	EUR 530.08 EUR 245.00 EUR 1712.80 EUR 637.60 EUR 1175.20 EUR 418.00 EUR 4132.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Uniprot ID: Q05928 Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. Form: Freeze-dried powder Predicted Molecular Mass (KD): 11.4kDa Isoelectric Point: Inquire
Description: Recombinant Mouse Betacellulin expressed in: E.coli

Betacellulin, murine (mouse)
RC238-23	Bio Basic	5ug	EUR 104.38
Product category: Proteins/Recombinant Proteins/Growth Factors

Human Betacellulin (BTC) ELISA Kit
abx570040-96tests	Abbexa	96 tests	EUR 668
Shipped within 5-12 working days.

Human Betacellulin (bTC) Protein (Active)
20-abx655563	Abbexa	EUR 1024.00 EUR 356.00 EUR 3376.00 EUR 1233.00 EUR 704.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-15 working days.

Human BTC(Betacellulin) ELISA Kit
EH0044	FN Test	96T	EUR 524.1
Detection range: 15.625-1000 pg/ml Uniprot ID: P35070 Alias: BTC(Betacellulin)/bTC
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml

Human Betacellulin (bTC) CLIA Kit
abx196482-96tests	Abbexa	96 tests	EUR 825
Shipped within 5-12 working days.

Human Betacellulin (BTC) CLIA Kit
20-abx491043	Abbexa	EUR 7973.00 EUR 4246.00 EUR 981.00	10 × 96 tests 5 × 96 tests 96 tests
Please enquire.

Human Betacellulin (BTC) ELISA Kit
abx250182-96tests	Abbexa	96 tests	EUR 668
Shipped within 5-12 working days.

Human Betacellulin (bTC) ELISA Kit
DLR-bTC-Hu-48T	DL Develop	48T	EUR 479
Should the Human Betacellulin (bTC) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Betacellulin (bTC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.

Human Betacellulin (bTC) ELISA Kit
DLR-bTC-Hu-96T	DL Develop	96T	EUR 621
Should the Human Betacellulin (bTC) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Betacellulin (bTC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.

Human Betacellulin, BTC ELISA Kit
DEIA089	Creative Diagnostics	10 plates	EUR 2605
Description: This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). It is developed for quantitative measurement of human BTC in serum, plasma and other biological fluids.

Betacellulin (bTC) Polyclonal Antibody (Human)
4-PAA112Hu01	Cloud-Clone	EUR 232.00 EUR 2285.00 EUR 574.00 EUR 289.00 EUR 208.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: bTC (Asp32~Tyr111) Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC)

Human Betacellulin/BTC ELISA Kit
LF-EK50996	Abfrontier	1×96T	EUR 648

Human Betacellulin (bTC) ELISA Kit
SEA112Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 4273.35
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Betacellulin (bTC) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Human Betacellulin (bTC) ELISA Kit
SEA112Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 439.57
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Betacellulin (bTC) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Human Betacellulin (bTC) ELISA Kit
SEA112Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 585.1
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Betacellulin (bTC) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Human Betacellulin (bTC) ELISA Kit
SEA112Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2332.95
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Betacellulin (bTC) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Human Betacellulin (bTC) ELISA Kit
4-SEA112Hu	Cloud-Clone	EUR 4324.00 EUR 2283.00 EUR 586.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Known also as Betacellulin elisa. Alternative names of the recognized antigen: Probetacellulin
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Betacellulin (bTC) in samples from Serum, plasma, tissue homogenates, cell lysates and other biological fluids. with no significant corss-reactivity with analogues from other species.

Human Betacellulin (bTC) ELISA Kit
RD-bTC-Hu-48Tests	Reddot Biotech	48 Tests	EUR 478

Human Betacellulin (bTC) ELISA Kit
RD-bTC-Hu-96Tests	Reddot Biotech	96 Tests	EUR 662

Human Betacellulin (bTC) ELISA Kit
RDR-bTC-Hu-48Tests	Reddot Biotech	48 Tests	EUR 500

Human Betacellulin (bTC) ELISA Kit
RDR-bTC-Hu-96Tests	Reddot Biotech	96 Tests	EUR 692

BTC Betacellulin Human Recombinant Protein
PROTP35070-1	BosterBio	Regular: 20ug	EUR 317
Description: Betacellulin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 80 amino acids and having a molecular mass of 9 kDa. ;Betacellulin Human Recombinant is purified by proprietary chromatographic techniques.

Rat Betacellulin (bTC) Protein
20-abx652663	Abbexa	EUR 578.00 EUR 258.00 EUR 1720.00 EUR 690.00 EUR 425.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-7 working days.

Cow Betacellulin (bTC) Protein
20-abx652665	Abbexa	EUR 648.00 EUR 272.00 EUR 1998.00 EUR 773.00 EUR 467.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-15 working days.

Chicken Betacellulin (bTC) Protein
20-abx652666	Abbexa	EUR 648.00 EUR 272.00 EUR 1998.00 EUR 773.00 EUR 467.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-15 working days.

Betacellulin (HEK293-expressed), Mouse
HY-P7142	MedChemExpress	50ug	EUR 533

Mouse Betacellulin (bTC) Protein
20-abx065525	Abbexa	EUR 732.00 EUR 286.00 EUR 2305.00 EUR 885.00 EUR 523.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-15 working days.

Betacellulin (bTC) Antibody (FITC)
20-abx274036	Abbexa	EUR 411.00 EUR 230.00 EUR 1135.00 EUR 551.00 EUR 314.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-15 working days.

Betacellulin (bTC) Antibody (Biotin)
20-abx271925	Abbexa	EUR 425.00 EUR 230.00 EUR 1191.00 EUR 578.00 EUR 328.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-15 working days.

Betacellulin (bTC) Antibody (Biotin)
20-abx272658	Abbexa	EUR 384.00 EUR 230.00 EUR 1052.00 EUR 523.00 EUR 300.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-15 working days.

Rat Betacellulin antiserum (Rabbit)
PAAQ1	GroPep	100 µl	EUR 299

Bovine Betacellulin antiserum (Rabbit)
PAAR1	GroPep	100 µl	EUR 299

Recombinant Murine Betacellulin Protein
PROTQ05928-1	BosterBio	20ug	EUR 317
Description: Betacellulin is an EGF-related polypeptide growth factor that signals through the EGF receptor. It is produced in several tissues, including the pancreas, small intestine, and in certain tumor cells. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. Betacellulin is initially synthesized as a glycosylated 32.0 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce the mature sequence. Recombinant murine Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein.

ELISA kit for Human Betacellulin/BTC
EK5454	SAB	96 tests	EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Betacellulin/BTC in samples from serum, plasma, tissue homogenates and other biological fluids.

Human Betacellulin/BTC PicoKine ELISA Kit
EK0980	BosterBio	96 wells	EUR 425
Description: For quantitative detection of human Betacellulin in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

ELISA kit for Human bTC (Betacellulin)
ELK1403	ELK Biotech	1 plate of 96 wells	EUR 432
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Betacellulin (bTC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Betacellulin (b Show more
Description: A sandwich ELISA kit for detection of Betacellulin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

CLIA kit for Human BTC (Betacellulin)
E-CL-H0444	Elabscience Biotech	1 plate of 96 wells	EUR 584
Gentaur's BTC CLIA kit utilizes the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BTC . Standards or samples are added to the micro CLIA plate wells and combined with the sp Show more
Description: A sandwich CLIA kit for quantitative measurement of Human BTC (Betacellulin) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human BTC (Betacellulin)
E-EL-H0570	Elabscience Biotech	1 plate of 96 wells	EUR 534
Gentaur's BTC ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BTC. Standards or samples are added to the micro ELISA plate wells and combined with the Show more
Description: A sandwich ELISA kit for quantitative measurement of Human BTC (Betacellulin) in samples from Serum, Plasma, Cell supernatant

Betacellulin (bTC) Polyclonal Antibody (Human), APC
4-PAA112Hu01-APC	Cloud-Clone	EUR 323.00 EUR 2969.00 EUR 836.00 EUR 409.00 EUR 210.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: bTC (Asp32~Tyr111) Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with APC.

Betacellulin (bTC) Polyclonal Antibody (Human), Biotinylated
4-PAA112Hu01-Biotin	Cloud-Clone	EUR 295.00 EUR 2235.00 EUR 671.00 EUR 358.00 EUR 212.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: bTC (Asp32~Tyr111) Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with Biotin.

Betacellulin (bTC) Polyclonal Antibody (Human), Cy3
4-PAA112Hu01-Cy3	Cloud-Clone	EUR 390.00 EUR 3917.00 EUR 1073.00 EUR 504.00 EUR 239.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: bTC (Asp32~Tyr111) Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with Cy3.

Betacellulin (bTC) Polyclonal Antibody (Human), FITC
4-PAA112Hu01-FITC	Cloud-Clone	EUR 279.00 EUR 2395.00 EUR 688.00 EUR 347.00 EUR 188.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: bTC (Asp32~Tyr111) Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with FITC.

Betacellulin (bTC) Polyclonal Antibody (Human), HRP
4-PAA112Hu01-HRP	Cloud-Clone	EUR 297.00 EUR 2589.00 EUR 741.00 EUR 371.00 EUR 199.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: bTC (Asp32~Tyr111) Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with HRP.

Betacellulin (bTC) Polyclonal Antibody (Human), PE
4-PAA112Hu01-PE	Cloud-Clone	EUR 279.00 EUR 2395.00 EUR 688.00 EUR 347.00 EUR 188.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: bTC (Asp32~Tyr111) Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with PE.

Monkey Betacellulin (BTC) ELISA Kit
abx359865-96tests	Abbexa	96 tests	EUR 825
Shipped within 5-12 working days.

Pig Betacellulin (BTC) ELISA Kit
abx361663-96tests	Abbexa	96 tests	EUR 825
Shipped within 5-12 working days.

Rabbit Betacellulin (BTC) ELISA Kit
abx363832-96tests	Abbexa	96 tests	EUR 825
Shipped within 5-12 working days.

Chicken Betacellulin (BTC) ELISA Kit
abx574267-96tests	Abbexa	96 tests	EUR 825
Shipped within 5-12 working days.

Mouse Betacellulin (BTC) ELISA Kit
abx574631-96tests	Abbexa	96 tests	EUR 668
Shipped within 5-12 working days.

Cow Betacellulin (BTC) ELISA Kit
abx575529-96tests	Abbexa	96 tests	EUR 911
Shipped within 5-12 working days.

Rat Betacellulin (BTC) ELISA Kit
abx576495-96tests	Abbexa	96 tests	EUR 825
Shipped within 5-12 working days.

Mouse Betacellulin (BTC) ELISA Kit
20-abx153708	Abbexa	EUR 6047.00 EUR 3228.00 EUR 754.00	10 × 96 tests 5 × 96 tests 96 tests
Shipped within 5-7 working days.

Mouse Betacellulin (BTC) CLIA Kit
20-abx491044	Abbexa	EUR 7378.00 EUR 3933.00 EUR 911.00	10 × 96 tests 5 × 96 tests 96 tests
Please enquire.

Rat Betacellulin (BTC) ELISA Kit
abx255590-96tests	Abbexa	96 tests	EUR 668
Shipped within 5-12 working days.

Mouse Betacellulin (BTC) ELISA Kit
abx253974-96tests	Abbexa	96 tests	EUR 668
Shipped within 5-12 working days.

Mouse Betacellulin (bTC) ELISA Kit
DLR-bTC-Mu-48T	DL Develop	48T	EUR 433
Should the Mouse Betacellulin (bTC) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Betacellulin (bTC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.

Mouse Betacellulin (bTC) ELISA Kit
DLR-bTC-Mu-96T	DL Develop	96T	EUR 558
Should the Mouse Betacellulin (bTC) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Betacellulin (bTC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.

Rat Betacellulin (bTC) ELISA Kit
DLR-bTC-Ra-48T	DL Develop	48T	EUR 508
Should the Rat Betacellulin (bTC) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Betacellulin (bTC) in samples from serum, plasma, tissue homogenates, cell lysates,cell culture supernates or other biological fluids.

Rat Betacellulin (bTC) ELISA Kit
DLR-bTC-Ra-96T	DL Develop	96T	EUR 661
Should the Rat Betacellulin (bTC) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Betacellulin (bTC) in samples from serum, plasma, tissue homogenates, cell lysates,cell culture supernates or other biological fluids.

BTC Betacellulin Bovine Recombinant Protein
PROTQ9TTC5	BosterBio	Regular: 20ug	EUR 317
Description: Betacellulin Bovine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 80 amino acids and having a molecular mass of 9003 Dalton. ;Betacellulin Bovine Recombinant is purified by proprietary chromatographic techniques.

Betacellulin (bTC) Polyclonal Antibody (Mouse)
4-PAA112Mu01	Cloud-Clone	EUR 214.00 EUR 1995.00 EUR 508.00 EUR 263.00 EUR 200.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: bTC (Asp32~Gln118) Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Betacellulin (bTC)

Betacellulin (bTC) Polyclonal Antibody (Rat)
4-PAA112Ra01	Cloud-Clone	EUR 243.00 EUR 2450.00 EUR 611.00 EUR 304.00 EUR 212.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: Inquire for antigen sequence. Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rat Betacellulin (bTC)

Mouse Betacellulin (bTC) ELISA Kit
SEA112Mu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 3710.48
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Betacellulin (bTC) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Mouse Betacellulin (bTC) ELISA Kit
SEA112Mu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 393.22
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Betacellulin (bTC) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Mouse Betacellulin (bTC) ELISA Kit
SEA112Mu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 518.88
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Betacellulin (bTC) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Mouse Betacellulin (bTC) ELISA Kit
SEA112Mu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2034.96
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Betacellulin (bTC) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Mouse Betacellulin (bTC) ELISA Kit
4-SEA112Mu	Cloud-Clone	EUR 3761.00 EUR 1985.00 EUR 519.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Known also as Betacellulin elisa. Alternative names of the recognized antigen: Probetacellulin
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Betacellulin (bTC) in samples from Serum, plasma, tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Betacellulin (bTC) ELISA Kit
RD-bTC-Mu-48Tests	Reddot Biotech	48 Tests	EUR 426

Mouse Betacellulin (bTC) ELISA Kit
RD-bTC-Mu-96Tests	Reddot Biotech	96 Tests	EUR 587

Rat Betacellulin (bTC) ELISA Kit
RD-bTC-Ra-48Tests	Reddot Biotech	48 Tests	EUR 511

Rat Betacellulin (bTC) ELISA Kit
RD-bTC-Ra-96Tests	Reddot Biotech	96 Tests	EUR 709

Mouse Betacellulin (bTC) ELISA Kit
RDR-bTC-Mu-48Tests	Reddot Biotech	48 Tests	EUR 445

Mouse Betacellulin (bTC) ELISA Kit
RDR-bTC-Mu-96Tests	Reddot Biotech	96 Tests	EUR 614

Rat Betacellulin (bTC) ELISA Kit
RDR-bTC-Ra-48Tests	Reddot Biotech	48 Tests	EUR 534

Rat Betacellulin (bTC) ELISA Kit
RDR-bTC-Ra-96Tests	Reddot Biotech	96 Tests	EUR 742

BTC Betacellulin Mouse Recombinant Protein
PROTQ05928	BosterBio	Regular: 20ug	EUR 317
Description: BTC Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 80 amino acids and having a molecular mass of 9.0kDa. ;The BTC is purified by proprietary chromatographic techniques.

Human Probetacellulin [Cleaved into: Betacellulin protein (BTC)
1-CSB-EP002853HU	Cusabio	EUR 380.00 EUR 214.00 EUR 1309.00 EUR 560.00 EUR 873.00 EUR 262.00	100ug 10ug 1MG 200ug 500ug 50ug
MW: 43.6 kDa Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human Probetacellulin [Cleaved into: Betacellulin protein(BTC) expressed in E.coli

Betacellulin (bTC) Polyclonal Antibody (Human), APC-Cy7
4-PAA112Hu01-APC-Cy7	Cloud-Clone	EUR 527.00 EUR 5818.00 EUR 1552.00 EUR 698.00 EUR 301.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: bTC (Asp32~Tyr111) Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with APC-Cy7.

BTC Betacellulin Human Recombinant Protein, His Tag
PROTP35070	BosterBio	Regular: 10ug	EUR 317
Description: BTC Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 101 amino acids (32-111) and having a molecular mass of 11.3 kDa.;BTC is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Betacellulin ELISA Kit (Human) : 96 Wells (OKAG00006)
OKAG00006	Aviva Systems Biology	96 Wells	EUR 596
Description: Description of target: This gene encodes a member of the epidermal growth factor (EGF) family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the secreted growth factor. A secreted form and a membrane-anchored form of this protein bind to multiple different EGF receptors. This protein promotes pancreatic cell proliferation and insulin secretion, as well as retinal vascular permeability. Mutations in this gene may be associated with type 2 diabetes in human patients.;Species reactivity: Human;Application: ELISA;Assay info: Quantitative Colorimentric Sandwich ELISA;Sensitivity: 8 pg/mL

Recombinant Human BTC/ Betacellulin Protein, Untagged, E.coli-100ug
QP10416-ec-100ug	EnQuireBio	100ug	EUR 988

Recombinant Human BTC/ Betacellulin Protein, Untagged, E.coli-20ug
QP10416-ec-20ug	EnQuireBio	20ug	EUR 290

Recombinant Human BTC/ Betacellulin Protein, Untagged, E.coli-250ug
QP10416-ec-250ug	EnQuireBio	250ug	EUR 1731

Recombinant Human BTC/ Betacellulin Protein, Untagged, E.coli-5ug
QP10416-ec-5ug	EnQuireBio	5ug	EUR 154

Recombinant Human BTC/ Betacellulin Protein, His, E.coli-100ug
QP5728-ec-100ug	EnQuireBio	100ug	EUR 418

The germinal heart (GC) is the positioning the place B cells endure clonal growth, affinity-based choice, and differentiation into reminiscence B cells or plasma cells. It has been tough to elucidate regulatory mechanisms for the dynamic GC B cell maturation and differentiation, partly as a result of experimental manipulation of GC B cells in vivo has been restricted and no in vitro system has been obtainable that resembles B cell response in GC. Here we describe the protocol for a tradition system named “induced GC B (iGB) tradition system” which might induce huge growth of B cells that exhibit GC B cell-like phenotype, and thus it mimics the GC response. This protocol might be helpful to elucidate the molecular mechanisms of GC B cell differentiation.