Co-culture of Human Stem Cell Derived Neurons and Oligodendrocyte Progenitor Cells

Co-culture of Human Stem Cell Derived Neurons and Oligodendrocyte Progenitor Cells

Crosstalk between neurons and oligodendrocytes is essential for correct mind functioning. Multiple co-culture strategies have been developed to check oligodendrocyte maturation, myelination or the impact of oligodendrocytes on neurons. However, most of these strategies comprise cells derived from animal fashions. In the present protocol, we co-culture human neurons with human oligodendrocytes. Neurons and oligodendrocyte precursor cells (OPCs) had been differentiated individually from pluripotent stem cells in keeping with beforehand revealed protocols. After the harvesting of cells from bone marrow by flushing the femoral diaphysis and enzymatic digestion of stomach and inguinal adipose tissues, MSCs are chosen by their adherence to the plastic tissue tradition dish.

To examine neuron-glia cross-talk, neurons and OPCs had been plated in co-culture mode in optimized situations for extra 28 days, and ready for OPC maturation and neuronal morphology evaluation. To our information, that is one of the primary neuron-OPC protocols containing all human cells. Specific neuronal abnormalities not noticed in mono-cultures of Tuberous Sclerosis Complex (TSC) neurons, turned obvious when TSC neurons had been co-cultured with TSC OPCs. These outcomes present that this co-culture system can be utilized to check human neuron-OPC interactive mechanisms concerned in well being and illness.

Since their discovery, mesenchymal stromal cells (MSCs) have obtained loads of consideration, primarily as a consequence of their self-renewal potential and multilineage differentiation capability. For these causes, MSCs are a great tool in cell biology and regenerative medication. In this text, we describe protocols to isolate MSCs from bone marrow (BM-MSCs) and adipose tissues (AT-MSCs), and strategies to tradition, characterize, and differentiate MSCs into osteoblasts, adipocytes, and chondrocytes. Within 7 days, MSCs attain 70% confluence and are prepared for use in subsequent experiments. The protocols described listed here are simple to carry out, cost-efficient, require minimal time, and yield a cell inhabitants wealthy in MSCs.

Method for Primary Epithelial Cell Culture from the Rat Choroid Plexus

 

The choroid plexus consists of a community of secretory epithelial cells localized all through the lateral, third and fourth ventricles of the mind. Cerebrospinal fluid (CSF) is generated by the choroid plexus and launched into the ventricular atmosphere. This biofluid accommodates an enriched supply of proteins, ions, and different signaling molecules for extracellular assist of neurons and glial cells inside the central nervous system. Given that different cells within the mind additionally launch elements into the CSF, in vitro investigations of choroid plexus operate are essential to isolate processes selectively occurring inside and launched from this tissue.

Here, we describe a protocol to isolate choroid plexus tissue from every of the ventricular areas, and the cell tradition situations required to assist development and upkeep of these epithelial cells. This approach permits for investigations of the practical significance of the choroid plexus, akin to for the examination of stimuli selling the discharge of development elements and extracellular vesicles (e.g., exosomes and microvesicles) from ventricle-specific choroid plexus epithelial cells.

Investigations into glial biology have contributed considerably in understanding the physiology and pathology of the nervous system. However, intricacies of the neuron-glial and glial-glial interactions in vivo current vital challenges whereas delineating the person cell-type contributions, thus making the in vitro strategies exceedingly related to check glial biology. However, acquiring optimum yield together with excessive purity has been difficult for microglial cultures. Here we current a easy protocol to determine enriched astroglial in addition to microglial cultures from the neonatal rat spinal twine. This methodology ends in extremely enriched astroglial and microglial cultures with maximal yield.

Co-culture of Human Stem Cell Derived Neurons and Oligodendrocyte Progenitor Cells

Quantification of Hepatitis B Virus Covalently Closed Circular DNA in Infected Cell Culture Models by Quantitative PCR

 

Persistence of the human hepatitis B virus (HBV) requires the upkeep of covalently closed round (ccc)DNA, the episomal genome reservoir in nuclei of contaminated hepatocytes. cccDNA elimination is a serious goal in future healing therapies presently underneath improvement. In cell tradition primarily based in vitro research, each hybridization- and amplification-based assays are presently used for cccDNA quantification. Southern blot, the present gold customary, is time-consuming and not sensible for a big quantity of samples. PCR-based strategies present restricted specificity when extreme HBV replicative intermediates are current. We have not too long ago developed a real-time quantitative PCR protocol, by which complete mobile DNA plus all types of viral DNA are extracted by silica column.

Subsequent incubation with T5 exonuclease effectively removes mobile DNA and all non-cccDNA types of viral DNA whereas cccDNA stays intact and can reliably be quantified by PCR. This methodology has been used for measuring kinetics of cccDNA accumulation in a number of in vitro an infection fashions and the impact of antivirals on cccDNA. It allowed detection of cccDNA in non-human cells (major macaque and swine hepatocytes, and so forth.) reconstituted with the HBV receptor, human sodium taurocholate cotransporting polypeptide (NTCP). Here we current an in depth protocol of this methodology, together with a piece flowchart, schematic diagram and illustrations on find out how to calculate “cccDNA copies per (contaminated) cell”.

anti-Betacellulin
YF-PA10526 50 ug
EUR 363
Description: Mouse polyclonal to Betacellulin
anti-Betacellulin
YF-PA10527 100 ul
EUR 403
Description: Rabbit polyclonal to Betacellulin
anti-Betacellulin
YF-PA10528 100 ug
EUR 403
Description: Rabbit polyclonal to Betacellulin
anti- Betacellulin antibody
FNab00883 100µg
EUR 505.25
  • Recommended dilution: WB: 1:500-1:2000
  • Immunogen: betacellulin
  • Uniprot ID: P35070
  • Gene ID: 685
  • Research Area: Cancer, Signal Transduction, Immunology
Description: Antibody raised against Betacellulin
Anti-Betacellulin antibody
PAab00883 100 ug
EUR 355
Betacellulin, Human
HY-P7005 10ug
EUR 211
Betacellulin, human
RC218-23 5ug
EUR 104.38
  • Product category: Proteins/Recombinant Proteins/Growth Factors
Anti-Betacellulin/Btc Antibody
A02171-1 100ug/vial
EUR 334
Rabbit Polyclonal antibody Anti-CRBN
Anti-CRBN 50 µg
EUR 349
Recombinant Human Betacellulin
7-00223 5µg Ask for price
Recombinant Human Betacellulin
7-00224 20µg Ask for price
Recombinant Human Betacellulin
7-00225 1mg Ask for price
Bovine Betacellulin
ARU020 20 µg
EUR 253
Bovine Betacellulin
ARU100 100 µg
EUR 346
Betacellulin, Mouse
HY-P7329 50ug
EUR 612
Betacellulin antibody
70R-BR013 50 ug
EUR 273
Description: Affinity purified Rabbit polyclonal Betacellulin antibody
Betacellulin protein
30R-AB028 20 ug
EUR 273
Description: Purified recombinant Human Betacellulin protein
Betacellulin (HEK293-expressed), Human
HY-P7328 10ug
EUR 234
Human Betacellulin (bTC) Protein
20-abx065524
  • EUR 648.00
  • EUR 272.00
  • EUR 1943.00
  • EUR 759.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-12 working days.
Recombinant Human Betacellulin Protein
PROTP35070-2 20ug
EUR 317
Description: Betacellulin is an EGF-related polypeptide growth factor that signals through the EGF receptor. It is produced in several tissues, including the pancreas, small intestine, and in certain tumor cells. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. Human Betacellulin is initially synthesized as a glycosylated 32.0 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce the mature sequence. Recombinant human Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein.
Polyclonal Goat anti-GST α-form
GST-ANTI-1 50 uL
EUR 280
Polyclonal Goat anti-GST μ-form
GST-ANTI-2 50 uL
EUR 280
Polyclonal Goat anti-GST p-form
GST-ANTI-3 50 uL
EUR 280
Betacellulin (BTC) Antibody
20-abx111218
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.
Betacellulin (bTC) Antibody
20-abx100739
  • EUR 342.00
  • EUR 133.00
  • EUR 982.00
  • EUR 495.00
  • EUR 286.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-12 working days.
Betacellulin (bTC) Antibody
20-abx100740
  • EUR 398.00
  • EUR 133.00
  • EUR 1107.00
  • EUR 537.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.
Betacellulin (bTC) Antibody
20-abx175568
  • EUR 1135.00
  • EUR 551.00
  • 1 mg
  • 200 ug
  • Please enquire.
Betacellulin (bTC) Antibody
20-abx175569
  • EUR 1177.00
  • EUR 578.00
  • 1 mg
  • 200 ug
  • Please enquire.
Betacellulin (bTC) Antibody
20-abx175570
  • EUR 1177.00
  • EUR 578.00
  • 1 mg
  • 200 ug
  • Please enquire.
Betacellulin (bTC) Antibody
20-abx175571
  • EUR 1288.00
  • EUR 620.00
  • 1 mg
  • 200 ug
  • Please enquire.
Betacellulin (BTC) Antibody
abx230883-100ug 100 ug
EUR 481
  • Shipped within 5-12 working days.
Betacellulin (bTC) Antibody
20-abx171411
  • EUR 787.00
  • EUR 411.00
  • 1 mg
  • 200 ug
  • Please enquire.
Betacellulin, Murine Recombinant
7119-10
EUR 196
Betacellulin, Murine Recombinant
7119-50
EUR 675
Recombinant Bovine Betacellulin
7-00226 5µg Ask for price
Recombinant Bovine Betacellulin
7-00227 20µg Ask for price
Recombinant Bovine Betacellulin
7-00228 1mg Ask for price
Recombinant Betacellulin (bTC)
4-RPA112Hu01
  • EUR 458.40
  • EUR 226.00
  • EUR 1444.00
  • EUR 548.00
  • EUR 996.00
  • EUR 370.00
  • EUR 3460.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P35070
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 10.2kDa
  • Isoelectric Point: Inquire
Description: Recombinant Human Betacellulin expressed in: E.coli
Recombinant Betacellulin (bTC)
4-RPA112Mu01
  • EUR 530.08
  • EUR 245.00
  • EUR 1712.80
  • EUR 637.60
  • EUR 1175.20
  • EUR 418.00
  • EUR 4132.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Q05928
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 11.4kDa
  • Isoelectric Point: Inquire
Description: Recombinant Mouse Betacellulin expressed in: E.coli
Betacellulin, murine (mouse)
RC238-23 5ug
EUR 104.38
  • Product category: Proteins/Recombinant Proteins/Growth Factors
Human Betacellulin (BTC) ELISA Kit
abx570040-96tests 96 tests
EUR 668
  • Shipped within 5-12 working days.
Human Betacellulin (bTC) Protein (Active)
20-abx655563
  • EUR 1024.00
  • EUR 356.00
  • EUR 3376.00
  • EUR 1233.00
  • EUR 704.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.
Human BTC(Betacellulin) ELISA Kit
EH0044 96T
EUR 524.1
  • Detection range: 15.625-1000 pg/ml
  • Uniprot ID: P35070
  • Alias: BTC(Betacellulin)/bTC
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml
Human Betacellulin (bTC) CLIA Kit
abx196482-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.
Human Betacellulin (BTC) CLIA Kit
20-abx491043
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Please enquire.
Human Betacellulin (BTC) ELISA Kit
abx250182-96tests 96 tests
EUR 668
  • Shipped within 5-12 working days.
Human Betacellulin (bTC) ELISA Kit
DLR-bTC-Hu-48T 48T
EUR 479
  • Should the Human Betacellulin (bTC) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Betacellulin (bTC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.
Human Betacellulin (bTC) ELISA Kit
DLR-bTC-Hu-96T 96T
EUR 621
  • Should the Human Betacellulin (bTC) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Betacellulin (bTC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.
Human Betacellulin, BTC ELISA Kit
DEIA089 10 plates
EUR 2605
Description: This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). It is developed for quantitative measurement of human BTC in serum, plasma and other biological fluids.
Betacellulin (bTC) Polyclonal Antibody (Human)
4-PAA112Hu01
  • EUR 232.00
  • EUR 2285.00
  • EUR 574.00
  • EUR 289.00
  • EUR 208.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: bTC (Asp32~Tyr111)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC)
Human Betacellulin/BTC ELISA Kit
LF-EK50996 1×96T
EUR 648
Human Betacellulin (bTC) ELISA Kit
SEA112Hu-10x96wellstestplate 10x96-wells test plate
EUR 4273.35
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Betacellulin (bTC) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Human Betacellulin (bTC) ELISA Kit
SEA112Hu-1x48wellstestplate 1x48-wells test plate
EUR 439.57
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Betacellulin (bTC) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Human Betacellulin (bTC) ELISA Kit
SEA112Hu-1x96wellstestplate 1x96-wells test plate
EUR 585.1
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Betacellulin (bTC) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Human Betacellulin (bTC) ELISA Kit
SEA112Hu-5x96wellstestplate 5x96-wells test plate
EUR 2332.95
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Betacellulin (bTC) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Human Betacellulin (bTC) ELISA Kit
4-SEA112Hu
  • EUR 4324.00
  • EUR 2283.00
  • EUR 586.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
  • Known also as Betacellulin elisa. Alternative names of the recognized antigen: Probetacellulin
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Betacellulin (bTC) in samples from Serum, plasma, tissue homogenates, cell lysates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Human Betacellulin (bTC) ELISA Kit
RD-bTC-Hu-48Tests 48 Tests
EUR 478
Human Betacellulin (bTC) ELISA Kit
RD-bTC-Hu-96Tests 96 Tests
EUR 662
Human Betacellulin (bTC) ELISA Kit
RDR-bTC-Hu-48Tests 48 Tests
EUR 500
Human Betacellulin (bTC) ELISA Kit
RDR-bTC-Hu-96Tests 96 Tests
EUR 692
BTC Betacellulin Human Recombinant Protein
PROTP35070-1 Regular: 20ug
EUR 317
Description: Betacellulin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 80 amino acids and having a molecular mass of 9 kDa. ;Betacellulin Human Recombinant is purified by proprietary chromatographic techniques.
Rat Betacellulin (bTC) Protein
20-abx652663
  • EUR 578.00
  • EUR 258.00
  • EUR 1720.00
  • EUR 690.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.
Cow Betacellulin (bTC) Protein
20-abx652665
  • EUR 648.00
  • EUR 272.00
  • EUR 1998.00
  • EUR 773.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.
Chicken Betacellulin (bTC) Protein
20-abx652666
  • EUR 648.00
  • EUR 272.00
  • EUR 1998.00
  • EUR 773.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.
Betacellulin (HEK293-expressed), Mouse
HY-P7142 50ug
EUR 533
Mouse Betacellulin (bTC) Protein
20-abx065525
  • EUR 732.00
  • EUR 286.00
  • EUR 2305.00
  • EUR 885.00
  • EUR 523.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.
Betacellulin (bTC) Antibody (FITC)
20-abx274036
  • EUR 411.00
  • EUR 230.00
  • EUR 1135.00
  • EUR 551.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.
Betacellulin (bTC) Antibody (Biotin)
20-abx271925
  • EUR 425.00
  • EUR 230.00
  • EUR 1191.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.
Betacellulin (bTC) Antibody (Biotin)
20-abx272658
  • EUR 384.00
  • EUR 230.00
  • EUR 1052.00
  • EUR 523.00
  • EUR 300.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.
Rat Betacellulin antiserum (Rabbit)
PAAQ1 100 µl
EUR 299
Bovine Betacellulin antiserum (Rabbit)
PAAR1 100 µl
EUR 299
Recombinant Murine Betacellulin Protein
PROTQ05928-1 20ug
EUR 317
Description: Betacellulin is an EGF-related polypeptide growth factor that signals through the EGF receptor. It is produced in several tissues, including the pancreas, small intestine, and in certain tumor cells. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. Betacellulin is initially synthesized as a glycosylated 32.0 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce the mature sequence. Recombinant murine Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein.
ELISA kit for Human Betacellulin/BTC
EK5454 96 tests
EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Betacellulin/BTC in samples from serum, plasma, tissue homogenates and other biological fluids.
Human Betacellulin/BTC PicoKine ELISA Kit
EK0980 96 wells
EUR 425
Description: For quantitative detection of human Betacellulin in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
ELISA kit for Human bTC (Betacellulin)
ELK1403 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Betacellulin (bTC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Betacellulin (b
  • Show more
Description: A sandwich ELISA kit for detection of Betacellulin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
CLIA kit for Human BTC (Betacellulin)
E-CL-H0444 1 plate of 96 wells
EUR 584
  • Gentaur's BTC CLIA kit utilizes the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BTC . Standards or samples are added to the micro CLIA plate wells and combined with the sp
  • Show more
Description: A sandwich CLIA kit for quantitative measurement of Human BTC (Betacellulin) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Human BTC (Betacellulin)
E-EL-H0570 1 plate of 96 wells
EUR 534
  • Gentaur's BTC ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BTC. Standards or samples are added to the micro ELISA plate wells and combined with the
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Human BTC (Betacellulin) in samples from Serum, Plasma, Cell supernatant
Betacellulin (bTC) Polyclonal Antibody (Human), APC
4-PAA112Hu01-APC
  • EUR 323.00
  • EUR 2969.00
  • EUR 836.00
  • EUR 409.00
  • EUR 210.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: bTC (Asp32~Tyr111)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with APC.
Betacellulin (bTC) Polyclonal Antibody (Human), Biotinylated
4-PAA112Hu01-Biotin
  • EUR 295.00
  • EUR 2235.00
  • EUR 671.00
  • EUR 358.00
  • EUR 212.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: bTC (Asp32~Tyr111)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with Biotin.
Betacellulin (bTC) Polyclonal Antibody (Human), Cy3
4-PAA112Hu01-Cy3
  • EUR 390.00
  • EUR 3917.00
  • EUR 1073.00
  • EUR 504.00
  • EUR 239.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: bTC (Asp32~Tyr111)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with Cy3.
Betacellulin (bTC) Polyclonal Antibody (Human), FITC
4-PAA112Hu01-FITC
  • EUR 279.00
  • EUR 2395.00
  • EUR 688.00
  • EUR 347.00
  • EUR 188.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: bTC (Asp32~Tyr111)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with FITC.
Betacellulin (bTC) Polyclonal Antibody (Human), HRP
4-PAA112Hu01-HRP
  • EUR 297.00
  • EUR 2589.00
  • EUR 741.00
  • EUR 371.00
  • EUR 199.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: bTC (Asp32~Tyr111)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with HRP.
Betacellulin (bTC) Polyclonal Antibody (Human), PE
4-PAA112Hu01-PE
  • EUR 279.00
  • EUR 2395.00
  • EUR 688.00
  • EUR 347.00
  • EUR 188.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: bTC (Asp32~Tyr111)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with PE.
Monkey Betacellulin (BTC) ELISA Kit
abx359865-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.
Pig Betacellulin (BTC) ELISA Kit
abx361663-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.
Rabbit Betacellulin (BTC) ELISA Kit
abx363832-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.
Chicken Betacellulin (BTC) ELISA Kit
abx574267-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.
Mouse Betacellulin (BTC) ELISA Kit
abx574631-96tests 96 tests
EUR 668
  • Shipped within 5-12 working days.
Cow Betacellulin (BTC) ELISA Kit
abx575529-96tests 96 tests
EUR 911
  • Shipped within 5-12 working days.
Rat Betacellulin (BTC) ELISA Kit
abx576495-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.
Mouse Betacellulin (BTC) ELISA Kit
20-abx153708
  • EUR 6047.00
  • EUR 3228.00
  • EUR 754.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-7 working days.
Mouse Betacellulin (BTC) CLIA Kit
20-abx491044
  • EUR 7378.00
  • EUR 3933.00
  • EUR 911.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Please enquire.
Rat Betacellulin (BTC) ELISA Kit
abx255590-96tests 96 tests
EUR 668
  • Shipped within 5-12 working days.
Mouse Betacellulin (BTC) ELISA Kit
abx253974-96tests 96 tests
EUR 668
  • Shipped within 5-12 working days.
Mouse Betacellulin (bTC) ELISA Kit
DLR-bTC-Mu-48T 48T
EUR 433
  • Should the Mouse Betacellulin (bTC) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Betacellulin (bTC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.
Mouse Betacellulin (bTC) ELISA Kit
DLR-bTC-Mu-96T 96T
EUR 558
  • Should the Mouse Betacellulin (bTC) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Betacellulin (bTC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.
Rat Betacellulin (bTC) ELISA Kit
DLR-bTC-Ra-48T 48T
EUR 508
  • Should the Rat Betacellulin (bTC) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Betacellulin (bTC) in samples from serum, plasma, tissue homogenates, cell lysates,cell culture supernates or other biological fluids.
Rat Betacellulin (bTC) ELISA Kit
DLR-bTC-Ra-96T 96T
EUR 661
  • Should the Rat Betacellulin (bTC) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Betacellulin (bTC) in samples from serum, plasma, tissue homogenates, cell lysates,cell culture supernates or other biological fluids.
BTC Betacellulin Bovine Recombinant Protein
PROTQ9TTC5 Regular: 20ug
EUR 317
Description: Betacellulin Bovine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 80 amino acids and having a molecular mass of 9003 Dalton. ;Betacellulin Bovine Recombinant is purified by proprietary chromatographic techniques.
Betacellulin (bTC) Polyclonal Antibody (Mouse)
4-PAA112Mu01
  • EUR 214.00
  • EUR 1995.00
  • EUR 508.00
  • EUR 263.00
  • EUR 200.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: bTC (Asp32~Gln118)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Betacellulin (bTC)
Betacellulin (bTC) Polyclonal Antibody (Rat)
4-PAA112Ra01
  • EUR 243.00
  • EUR 2450.00
  • EUR 611.00
  • EUR 304.00
  • EUR 212.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Inquire for antigen sequence.
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rat Betacellulin (bTC)
Mouse Betacellulin (bTC) ELISA Kit
SEA112Mu-10x96wellstestplate 10x96-wells test plate
EUR 3710.48
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Betacellulin (bTC) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Mouse Betacellulin (bTC) ELISA Kit
SEA112Mu-1x48wellstestplate 1x48-wells test plate
EUR 393.22
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Betacellulin (bTC) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Mouse Betacellulin (bTC) ELISA Kit
SEA112Mu-1x96wellstestplate 1x96-wells test plate
EUR 518.88
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Betacellulin (bTC) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Mouse Betacellulin (bTC) ELISA Kit
SEA112Mu-5x96wellstestplate 5x96-wells test plate
EUR 2034.96
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Betacellulin (bTC) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Mouse Betacellulin (bTC) ELISA Kit
4-SEA112Mu
  • EUR 3761.00
  • EUR 1985.00
  • EUR 519.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
  • Known also as Betacellulin elisa. Alternative names of the recognized antigen: Probetacellulin
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Betacellulin (bTC) in samples from Serum, plasma, tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.
Mouse Betacellulin (bTC) ELISA Kit
RD-bTC-Mu-48Tests 48 Tests
EUR 426
Mouse Betacellulin (bTC) ELISA Kit
RD-bTC-Mu-96Tests 96 Tests
EUR 587
Rat Betacellulin (bTC) ELISA Kit
RD-bTC-Ra-48Tests 48 Tests
EUR 511
Rat Betacellulin (bTC) ELISA Kit
RD-bTC-Ra-96Tests 96 Tests
EUR 709
Mouse Betacellulin (bTC) ELISA Kit
RDR-bTC-Mu-48Tests 48 Tests
EUR 445
Mouse Betacellulin (bTC) ELISA Kit
RDR-bTC-Mu-96Tests 96 Tests
EUR 614
Rat Betacellulin (bTC) ELISA Kit
RDR-bTC-Ra-48Tests 48 Tests
EUR 534
Rat Betacellulin (bTC) ELISA Kit
RDR-bTC-Ra-96Tests 96 Tests
EUR 742
BTC Betacellulin Mouse Recombinant Protein
PROTQ05928 Regular: 20ug
EUR 317
Description: BTC Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 80 amino acids and having a molecular mass of 9.0kDa. ;The BTC is purified by proprietary chromatographic techniques.
Human Probetacellulin [Cleaved into: Betacellulin protein (BTC)
1-CSB-EP002853HU
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 43.6 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human Probetacellulin [Cleaved into: Betacellulin protein(BTC) expressed in E.coli
Betacellulin (bTC) Polyclonal Antibody (Human), APC-Cy7
4-PAA112Hu01-APC-Cy7
  • EUR 527.00
  • EUR 5818.00
  • EUR 1552.00
  • EUR 698.00
  • EUR 301.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: bTC (Asp32~Tyr111)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with APC-Cy7.
BTC Betacellulin Human Recombinant Protein, His Tag
PROTP35070 Regular: 10ug
EUR 317
Description: BTC Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 101 amino acids (32-111) and having a molecular mass of 11.3 kDa.;BTC is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Betacellulin ELISA Kit (Human) : 96 Wells (OKAG00006)
OKAG00006 96 Wells
EUR 596
Description: Description of target: This gene encodes a member of the epidermal growth factor (EGF) family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the secreted growth factor. A secreted form and a membrane-anchored form of this protein bind to multiple different EGF receptors. This protein promotes pancreatic cell proliferation and insulin secretion, as well as retinal vascular permeability. Mutations in this gene may be associated with type 2 diabetes in human patients.;Species reactivity: Human;Application: ELISA;Assay info: Quantitative Colorimentric Sandwich ELISA;Sensitivity: 8 pg/mL
Recombinant Human BTC/ Betacellulin Protein, Untagged, E.coli-100ug
QP10416-ec-100ug 100ug
EUR 988
Recombinant Human BTC/ Betacellulin Protein, Untagged, E.coli-20ug
QP10416-ec-20ug 20ug
EUR 290
Recombinant Human BTC/ Betacellulin Protein, Untagged, E.coli-250ug
QP10416-ec-250ug 250ug
EUR 1731
Recombinant Human BTC/ Betacellulin Protein, Untagged, E.coli-5ug
QP10416-ec-5ug 5ug
EUR 154
Recombinant Human BTC/ Betacellulin Protein, His, E.coli-100ug
QP5728-ec-100ug 100ug
EUR 418

The germinal heart (GC) is the positioning the place B cells endure clonal growth, affinity-based choice, and differentiation into reminiscence B cells or plasma cells. It has been tough to elucidate regulatory mechanisms for the dynamic GC B cell maturation and differentiation, partly as a result of experimental manipulation of GC B cells in vivo has been restricted and no in vitro system has been obtainable that resembles B cell response in GC. Here we describe the protocol for a tradition system named “induced GC B (iGB) tradition system” which might induce huge growth of B cells that exhibit GC B cell-like phenotype, and thus it mimics the GC response. This protocol might be helpful to elucidate the molecular mechanisms of GC B cell differentiation.