Co-culture of Human Stem Cell Derived Neurons and Oligodendrocyte Progenitor Cells

Co-culture of Human Stem Cell Derived Neurons and Oligodendrocyte Progenitor Cells

Crosstalk between neurons and oligodendrocytes is essential for correct mind functioning. Multiple co-culture strategies have been developed to check oligodendrocyte maturation, myelination or the impact of oligodendrocytes on neurons. However, most of these strategies comprise cells derived from animal fashions. In the present protocol, we co-culture human neurons with human oligodendrocytes. Neurons and oligodendrocyte precursor cells (OPCs) had been differentiated individually from pluripotent stem cells in keeping with beforehand revealed protocols. After the harvesting of cells from bone marrow by flushing the femoral diaphysis and enzymatic digestion of stomach and inguinal adipose tissues, MSCs are chosen by their adherence to the plastic tissue tradition dish.

To examine neuron-glia cross-talk, neurons and OPCs had been plated in co-culture mode in optimized situations for extra 28 days, and ready for OPC maturation and neuronal morphology evaluation. To our information, that is one of the primary neuron-OPC protocols containing all human cells. Specific neuronal abnormalities not noticed in mono-cultures of Tuberous Sclerosis Complex (TSC) neurons, turned obvious when TSC neurons had been co-cultured with TSC OPCs. These outcomes present that this co-culture system can be utilized to check human neuron-OPC interactive mechanisms concerned in well being and illness.

Since their discovery, mesenchymal stromal cells (MSCs) have obtained loads of consideration, primarily as a consequence of their self-renewal potential and multilineage differentiation capability. For these causes, MSCs are a great tool in cell biology and regenerative medication. In this text, we describe protocols to isolate MSCs from bone marrow (BM-MSCs) and adipose tissues (AT-MSCs), and strategies to tradition, characterize, and differentiate MSCs into osteoblasts, adipocytes, and chondrocytes. Within 7 days, MSCs attain 70% confluence and are prepared for use in subsequent experiments. The protocols described listed here are simple to carry out, cost-efficient, require minimal time, and yield a cell inhabitants wealthy in MSCs.

Method for Primary Epithelial Cell Culture from the Rat Choroid Plexus

 

The choroid plexus consists of a community of secretory epithelial cells localized all through the lateral, third and fourth ventricles of the mind. Cerebrospinal fluid (CSF) is generated by the choroid plexus and launched into the ventricular atmosphere. This biofluid accommodates an enriched supply of proteins, ions, and different signaling molecules for extracellular assist of neurons and glial cells inside the central nervous system. Given that different cells within the mind additionally launch elements into the CSF, in vitro investigations of choroid plexus operate are essential to isolate processes selectively occurring inside and launched from this tissue.

Here, we describe a protocol to isolate choroid plexus tissue from every of the ventricular areas, and the cell tradition situations required to assist development and upkeep of these epithelial cells. This approach permits for investigations of the practical significance of the choroid plexus, akin to for the examination of stimuli selling the discharge of development elements and extracellular vesicles (e.g., exosomes and microvesicles) from ventricle-specific choroid plexus epithelial cells.

Investigations into glial biology have contributed considerably in understanding the physiology and pathology of the nervous system. However, intricacies of the neuron-glial and glial-glial interactions in vivo current vital challenges whereas delineating the person cell-type contributions, thus making the in vitro strategies exceedingly related to check glial biology. However, acquiring optimum yield together with excessive purity has been difficult for microglial cultures. Here we current a easy protocol to determine enriched astroglial in addition to microglial cultures from the neonatal rat spinal twine. This methodology ends in extremely enriched astroglial and microglial cultures with maximal yield.

Co-culture of Human Stem Cell Derived Neurons and Oligodendrocyte Progenitor Cells

Quantification of Hepatitis B Virus Covalently Closed Circular DNA in Infected Cell Culture Models by Quantitative PCR

 

Persistence of the human hepatitis B virus (HBV) requires the upkeep of covalently closed round (ccc)DNA, the episomal genome reservoir in nuclei of contaminated hepatocytes. cccDNA elimination is a serious goal in future healing therapies presently underneath improvement. In cell tradition primarily based in vitro research, each hybridization- and amplification-based assays are presently used for cccDNA quantification. Southern blot, the present gold customary, is time-consuming and not sensible for a big quantity of samples. PCR-based strategies present restricted specificity when extreme HBV replicative intermediates are current. We have not too long ago developed a real-time quantitative PCR protocol, by which complete mobile DNA plus all types of viral DNA are extracted by silica column.

Subsequent incubation with T5 exonuclease effectively removes mobile DNA and all non-cccDNA types of viral DNA whereas cccDNA stays intact and can reliably be quantified by PCR. This methodology has been used for measuring kinetics of cccDNA accumulation in a number of in vitro an infection fashions and the impact of antivirals on cccDNA. It allowed detection of cccDNA in non-human cells (major macaque and swine hepatocytes, and so forth.) reconstituted with the HBV receptor, human sodium taurocholate cotransporting polypeptide (NTCP). Here we current an in depth protocol of this methodology, together with a piece flowchart, schematic diagram and illustrations on find out how to calculate “cccDNA copies per (contaminated) cell”.

anti-Betacellulin

YF-PA10528 100 ug
EUR 403
Description: Rabbit polyclonal to Betacellulin

anti- Betacellulin antibody

FNab00883 100µg
EUR 505.25
Description: Antibody raised against Betacellulin

Anti-Betacellulin antibody

PAab00883 100 ug
EUR 355

Betacellulin, Human

HY-P7005 10ug
EUR 211

Betacellulin, human

RC218-23 5ug
EUR 104.38

Anti-Betacellulin/Btc Antibody

A02171-1 100ug/vial
EUR 334

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

Recombinant Human Betacellulin

7-00223 5µg Ask for price

Recombinant Human Betacellulin

7-00224 20µg Ask for price

Recombinant Human Betacellulin

7-00225 1mg Ask for price

Betacellulin, Mouse

HY-P7329 50ug
EUR 612

Bovine Betacellulin

ARU020 20 µg
EUR 253

Bovine Betacellulin

ARU100 100 µg
EUR 346

Betacellulin antibody

70R-BR013 50 ug
EUR 273
Description: Affinity purified Rabbit polyclonal Betacellulin antibody

Betacellulin protein

30R-AB028 20 ug
EUR 273
Description: Purified recombinant Human Betacellulin protein

Betacellulin (HEK293-expressed), Human

HY-P7328 10ug
EUR 234

Human Betacellulin (bTC) Protein

20-abx065524
  • EUR 648.00
  • EUR 272.00
  • EUR 1943.00
  • EUR 759.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Recombinant Human Betacellulin Protein

PROTP35070-2 20ug
EUR 317
Description: Betacellulin is an EGF-related polypeptide growth factor that signals through the EGF receptor. It is produced in several tissues, including the pancreas, small intestine, and in certain tumor cells. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. Human Betacellulin is initially synthesized as a glycosylated 32.0 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce the mature sequence. Recombinant human Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein.

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

Betacellulin (BTC) Antibody

20-abx111218
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Betacellulin (bTC) Antibody

20-abx100739
  • EUR 342.00
  • EUR 133.00
  • EUR 982.00
  • EUR 495.00
  • EUR 286.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Betacellulin (bTC) Antibody

20-abx100740
  • EUR 398.00
  • EUR 133.00
  • EUR 1107.00
  • EUR 537.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Recombinant Bovine Betacellulin

7-00226 5µg Ask for price

Recombinant Bovine Betacellulin

7-00227 20µg Ask for price

Recombinant Bovine Betacellulin

7-00228 1mg Ask for price

Betacellulin (bTC) Antibody

20-abx171411
  • EUR 787.00
  • EUR 411.00
  • 1 mg
  • 200 ug

Betacellulin (BTC) Antibody

abx230883-100ug 100 ug
EUR 481

Betacellulin (bTC) Antibody

20-abx175568
  • EUR 1135.00
  • EUR 551.00
  • 1 mg
  • 200 ug

Betacellulin (bTC) Antibody

20-abx175569
  • EUR 1177.00
  • EUR 578.00
  • 1 mg
  • 200 ug

Betacellulin (bTC) Antibody

20-abx175570
  • EUR 1177.00
  • EUR 578.00
  • 1 mg
  • 200 ug

Betacellulin (bTC) Antibody

20-abx175571
  • EUR 1288.00
  • EUR 620.00
  • 1 mg
  • 200 ug

Betacellulin, murine (mouse)

RC238-23 5ug
EUR 104.38

Recombinant Betacellulin (bTC)

4-RPA112Hu01
  • EUR 458.40
  • EUR 226.00
  • EUR 1444.00
  • EUR 548.00
  • EUR 996.00
  • EUR 370.00
  • EUR 3460.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Betacellulin expressed in: E.coli

Recombinant Betacellulin (bTC)

4-RPA112Mu01
  • EUR 530.08
  • EUR 245.00
  • EUR 1712.80
  • EUR 637.60
  • EUR 1175.20
  • EUR 418.00
  • EUR 4132.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Betacellulin expressed in: E.coli

Betacellulin, Murine Recombinant

7119-10
EUR 196

Betacellulin, Murine Recombinant

7119-50
EUR 675

Betacellulin Recombinant Protein

40-192-0005mg 0.005 mg
EUR 259.25
Description: Betacellulin is an EGF-related polypeptide growth factor that signals through the EGF receptor. It is produced in several tissues, including the pancreas, small intestine, and in certain tumor cells. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. Human Betacellulin is initially synthesized as a glycosylated 32.0 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce the mature sequence. Recombinant human Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein.

Betacellulin Recombinant Protein

40-192-002mg 0.02 mg
EUR 364.25
Description: Betacellulin is an EGF-related polypeptide growth factor that signals through the EGF receptor. It is produced in several tissues, including the pancreas, small intestine, and in certain tumor cells. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. Human Betacellulin is initially synthesized as a glycosylated 32.0 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce the mature sequence. Recombinant human Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein.

Betacellulin Recombinant Protein

40-578-0005mg 0.005 mg
EUR 259.25
Description: Betacellulin is an EGF-related polypeptide growth factor that signals through the EGF receptor. It is produced in several tissues, including the pancreas, small intestine, and in certain tumor cells. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. Betacellulin is initially synthesized as a glycosylated 32.0 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce the mature sequence. Recombinant murine Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein.

Betacellulin Recombinant Protein

40-578-002mg 0.02 mg
EUR 364.25
Description: Betacellulin is an EGF-related polypeptide growth factor that signals through the EGF receptor. It is produced in several tissues, including the pancreas, small intestine, and in certain tumor cells. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. Betacellulin is initially synthesized as a glycosylated 32.0 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce the mature sequence. Recombinant murine Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein.

Betacellulin Recombinant Protein

96-830 0.05 mg
EUR 595.25
Description: Probetacellulin can be cleaved into the Betacellulin (BTC) that is a single-pass type I membrane protein containing 1 EGF-like domain. Predominantly expressed in pancreas and small intestine,Betacellulin (BTC) is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. As a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells, Betacellulin (BTC) is also the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Betacellulin (BTC) was originally identified as a growth-promoting factor in mouse pancreatic beta-cell carcinoma cell line and has since been identified in humans. The amino acid sequence of mature mBTC is 82.5%, identical with that of human BTC (hBTC), and both exhibit significant overall similarity with other members of the EGF family.

Betacellulin Recombinant Protein

91-592 0.05 mg
EUR 542.75
Description: Mouse Betacellulin is a single type I membrane protein which belongs to the EGF family of cytokines. EGF family has many members including EGF, TGF-a, Amphiregulin, HB-EGF, Epiregulin, Tomoregulin and the Neuregulins. Betacellulin is characterised by a six-cysteine consensus motif that forms three intra-molecular disulfide bonds crucial for binding the ErbB receptor family. Betacellulin is expressed in several tissues and tumor cells including kidney, uterus, liver, pancreas and small intestine. Betacellulin binds and activates ErbB-1 and ErbB-4 homodimers. Betacellulin is thought to play a role in the differentiation of pancreatic beta cells.Human and mouse mature BTC protein are 80% identical at the amino acid sequence level. Betacellulin is involved in many biological processes such as stimulating gastrointestinal growth. It is proteolytically processed from a larger membrane-anchored precursor and is a potent mitogen for a wide variety of cell types.

Human Betacellulin (BTC) ELISA Kit

abx570040-96tests 96 tests
EUR 668

Human Betacellulin, BTC ELISA Kit

DEIA089 10 plates
EUR 2605
Description: This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). It is developed for quantitative measurement of human BTC in serum, plasma and other biological fluids.

Human Betacellulin (BTC) CLIA Kit

20-abx491043
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Betacellulin (bTC) CLIA Kit

abx196482-96tests 96 tests
EUR 825

Human Betacellulin (BTC) ELISA Kit

abx250182-96tests 96 tests
EUR 668

Human Betacellulin (bTC) ELISA Kit

DLR-bTC-Hu-48T 48T
EUR 479
Description: A sandwich quantitative ELISA assay kit for detection of Human Betacellulin (bTC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.

Human Betacellulin (bTC) ELISA Kit

DLR-bTC-Hu-96T 96T
EUR 621
Description: A sandwich quantitative ELISA assay kit for detection of Human Betacellulin (bTC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.

Human Betacellulin (bTC) ELISA Kit

RD-bTC-Hu-48Tests 48 Tests
EUR 478

Human Betacellulin (bTC) ELISA Kit

RD-bTC-Hu-96Tests 96 Tests
EUR 662

BTC Betacellulin Human Recombinant Protein

PROTP35070-1 Regular: 20ug
EUR 317
Description: Betacellulin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 80 amino acids and having a molecular mass of 9 kDa. ;Betacellulin Human Recombinant is purified by proprietary chromatographic techniques.

Human Betacellulin (bTC) ELISA Kit

RDR-bTC-Hu-48Tests 48 Tests
EUR 500

Human Betacellulin (bTC) ELISA Kit

RDR-bTC-Hu-96Tests 96 Tests
EUR 692

Human Betacellulin (bTC) Protein (Active)

20-abx655563
  • EUR 1024.00
  • EUR 356.00
  • EUR 3376.00
  • EUR 1233.00
  • EUR 704.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human BTC(Betacellulin) ELISA Kit

EH0044 96T
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml

Betacellulin (bTC) Polyclonal Antibody (Human)

4-PAA112Hu01
  • EUR 232.00
  • EUR 2285.00
  • EUR 574.00
  • EUR 289.00
  • EUR 208.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC)

Human Betacellulin (bTC) ELISA Kit

SEA112Hu-10x96wellstestplate 10x96-wells test plate
EUR 4273.35
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Human Betacellulin (bTC) ELISA Kit

SEA112Hu-1x48wellstestplate 1x48-wells test plate
EUR 439.57
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Human Betacellulin (bTC) ELISA Kit

SEA112Hu-1x96wellstestplate 1x96-wells test plate
EUR 585.1
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Human Betacellulin (bTC) ELISA Kit

SEA112Hu-5x96wellstestplate 5x96-wells test plate
EUR 2332.95
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Betacellulin (bTC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Human Betacellulin (bTC) ELISA Kit

4-SEA112Hu
  • EUR 4324.00
  • EUR 2283.00
  • EUR 586.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Betacellulin (bTC) in samples from Serum, plasma, tissue homogenates, cell lysates and other biological fluids. with no significant corss-reactivity with analogues from other species.

Human Betacellulin/BTC ELISA Kit

LF-EK50996 1×96T
EUR 648

Betacellulin (HEK293-expressed), Mouse

HY-P7142 50ug
EUR 533

Rat Betacellulin antiserum (Rabbit)

PAAQ1 100 µl
EUR 299

Bovine Betacellulin antiserum (Rabbit)

PAAR1 100 µl
EUR 299

Mouse Betacellulin (bTC) Protein

20-abx065525
  • EUR 732.00
  • EUR 286.00
  • EUR 2305.00
  • EUR 885.00
  • EUR 523.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Betacellulin (bTC) Antibody (FITC)

20-abx274036
  • EUR 411.00
  • EUR 230.00
  • EUR 1135.00
  • EUR 551.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Betacellulin (bTC) Antibody (Biotin)

20-abx271925
  • EUR 425.00
  • EUR 230.00
  • EUR 1191.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Betacellulin (bTC) Antibody (Biotin)

20-abx272658
  • EUR 384.00
  • EUR 230.00
  • EUR 1052.00
  • EUR 523.00
  • EUR 300.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Recombinant Murine Betacellulin Protein

PROTQ05928-1 20ug
EUR 317
Description: Betacellulin is an EGF-related polypeptide growth factor that signals through the EGF receptor. It is produced in several tissues, including the pancreas, small intestine, and in certain tumor cells. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. Betacellulin is initially synthesized as a glycosylated 32.0 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce the mature sequence. Recombinant murine Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein.

Rat Betacellulin (bTC) Protein

20-abx652663
  • EUR 578.00
  • EUR 258.00
  • EUR 1720.00
  • EUR 690.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Cow Betacellulin (bTC) Protein

20-abx652665
  • EUR 648.00
  • EUR 272.00
  • EUR 1998.00
  • EUR 773.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Chicken Betacellulin (bTC) Protein

20-abx652666
  • EUR 648.00
  • EUR 272.00
  • EUR 1998.00
  • EUR 773.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

ELISA kit for Human bTC (Betacellulin)

ELK1403 1 plate of 96 wells
EUR 432
Description: A sandwich ELISA kit for detection of Betacellulin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Human BTC (Betacellulin)

E-EL-H0570 1 plate of 96 wells
EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Human BTC (Betacellulin) in samples from Serum, Plasma, Cell supernatant

Human Betacellulin/BTC PicoKine ELISA Kit

EK0980 96 wells
EUR 425
Description: For quantitative detection of human Betacellulin in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

CLIA kit for Human BTC (Betacellulin)

E-CL-H0444 1 plate of 96 wells
EUR 584
Description: A sandwich CLIA kit for quantitative measurement of Human BTC (Betacellulin) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human Betacellulin/BTC

EK5454 96 tests
EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Betacellulin/BTC in samples from serum, plasma, tissue homogenates and other biological fluids.

Betacellulin (bTC) Polyclonal Antibody (Human), APC

4-PAA112Hu01-APC
  • EUR 323.00
  • EUR 2969.00
  • EUR 836.00
  • EUR 409.00
  • EUR 210.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with APC.

Betacellulin (bTC) Polyclonal Antibody (Human), Biotinylated

4-PAA112Hu01-Biotin
  • EUR 295.00
  • EUR 2235.00
  • EUR 671.00
  • EUR 358.00
  • EUR 212.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with Biotin.

Betacellulin (bTC) Polyclonal Antibody (Human), Cy3

4-PAA112Hu01-Cy3
  • EUR 390.00
  • EUR 3917.00
  • EUR 1073.00
  • EUR 504.00
  • EUR 239.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with Cy3.

Betacellulin (bTC) Polyclonal Antibody (Human), FITC

4-PAA112Hu01-FITC
  • EUR 279.00
  • EUR 2395.00
  • EUR 688.00
  • EUR 347.00
  • EUR 188.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with FITC.

Betacellulin (bTC) Polyclonal Antibody (Human), HRP

4-PAA112Hu01-HRP
  • EUR 297.00
  • EUR 2589.00
  • EUR 741.00
  • EUR 371.00
  • EUR 199.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with HRP.

Betacellulin (bTC) Polyclonal Antibody (Human), PE

4-PAA112Hu01-PE
  • EUR 279.00
  • EUR 2395.00
  • EUR 688.00
  • EUR 347.00
  • EUR 188.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Betacellulin (bTC). This antibody is labeled with PE.

Mouse Betacellulin (BTC) ELISA Kit

20-abx153708
  • EUR 6047.00
  • EUR 3228.00
  • EUR 754.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Chicken Betacellulin (BTC) ELISA Kit

abx574267-96tests 96 tests
EUR 825

Mouse Betacellulin (BTC) ELISA Kit

abx574631-96tests 96 tests
EUR 668

Cow Betacellulin (BTC) ELISA Kit

abx575529-96tests 96 tests
EUR 911

Rat Betacellulin (BTC) ELISA Kit

abx576495-96tests 96 tests
EUR 825

Mouse Betacellulin (BTC) CLIA Kit

20-abx491044
  • EUR 7378.00
  • EUR 3933.00
  • EUR 911.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Betacellulin (BTC) ELISA Kit

abx255590-96tests 96 tests
EUR 668

Mouse Betacellulin (BTC) ELISA Kit

abx253974-96tests 96 tests
EUR 668

Pig Betacellulin (BTC) ELISA Kit

abx361663-96tests 96 tests
EUR 825

The germinal heart (GC) is the positioning the place B cells endure clonal growth, affinity-based choice, and differentiation into reminiscence B cells or plasma cells. It has been tough to elucidate regulatory mechanisms for the dynamic GC B cell maturation and differentiation, partly as a result of experimental manipulation of GC B cells in vivo has been restricted and no in vitro system has been obtainable that resembles B cell response in GC. Here we describe the protocol for a tradition system named “induced GC B (iGB) tradition system” which might induce huge growth of B cells that exhibit GC B cell-like phenotype, and thus it mimics the GC response. This protocol might be helpful to elucidate the molecular mechanisms of GC B cell differentiation.