Culture Cells

Femtosecond pulsed laser microscopy: a new tool to assess the in vitro delivered dose of carbon nanotubes in cell culture experiments

Femtosecond pulsed laser microscopy: a new tool to assess the in vitro delivered dose of carbon nanotubes in cell culture experiments

In vitro fashions are extensively used in nanotoxicology. In these assays, a cautious documentation of the fraction of nanomaterials that reaches the cells, i.e. the in vitro delivered dose, is a essential factor for the interpretation of the information. The in vitro delivered dose might be measured by quantifying the quantity of materials in contact …

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3D printing novel in vitro cancer cell culture model systems for lung cancer stem cell study

3D printing novel in vitro cancer cell culture model systems for lung cancer stem cell study

Two-dimensional (2D) in vitro cell cultures and laboratory animals have been used historically because the gold-standard preclinical cancer model systems. However, for cancer stem cell (CSC) research, they exhibit notable limitations on simulating native setting, which depreciate their translatability for medical improvement functions. In this study, totally different three-dimensional (3D) printing platforms have been used …

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Influence of Bacterial Culture Medium on Peptidoglycan Binding of Cell Wall Lytic Enzymes

Influence of Bacterial Culture Medium on Peptidoglycan Binding of Cell Wall Lytic Enzymes

The bacteriolysin lysostaphin (Lst) and endolysin PlyPH are potent modular lytic enzymes with exercise in opposition to clinically-relevant Gram-positive Staphylococcus aureus and Bacillus cereus, respectively. Both enzymes possess an N-terminal catalytic area and C-terminal binding area, with the latter conferring important enzyme specificity. Lst and PlyPH present diminished exercise within the presence of bacterial growth-supporting …

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Co-culture of Human Stem Cell Derived Neurons and Oligodendrocyte Progenitor Cells

Co-culture of Human Stem Cell Derived Neurons and Oligodendrocyte Progenitor Cells

Crosstalk between neurons and oligodendrocytes is essential for correct mind functioning. Multiple co-culture strategies have been developed to check oligodendrocyte maturation, myelination or the impact of oligodendrocytes on neurons. However, most of these strategies comprise cells derived from animal fashions. In the present protocol, we co-culture human neurons with human oligodendrocytes. Neurons and oligodendrocyte precursor …

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Quantifying HIV-1-Mediated Gut CD4 + T Cell Deathin the Lamina Propria Aggregate Culture (LPAC) Model

Quantifying HIV-1-Mediated Gut CD4 + T Cell Deathin the Lamina Propria Aggregate Culture (LPAC) Model

Gut CD4 T cells are main targets of HIV-1 and are massively depleted early throughout an infection. To higher perceive the mechanisms governing HIV-1-mediated CD4 T cell loss of life, we developed the physiologically-relevant Lamina Propria Aggregate Culture (LPAC) mannequin. The LPAC mannequin is right for learning CD4 T cell loss of life induced by …

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