Quantifying HIV-1-Mediated Gut CD4 + T Cell Deathin the Lamina Propria Aggregate Culture (LPAC) Model

Quantifying HIV-1-Mediated Gut CD4 + T Cell Deathin the Lamina Propria Aggregate Culture (LPAC) Model

Gut CD4 T cells are main targets of HIV-1 and are massively depleted early throughout an infection. To higher perceive the mechanisms governing HIV-1-mediated CD4 T cell loss of life, we developed the physiologically-relevant Lamina Propria Aggregate Culture (LPAC) mannequin. The LPAC mannequin is right for learning CD4 T cell loss of life induced by clinically-relevant Transmitted/Founder (TF) HIV-1 strains and can also be appropriate for learning how enteric microbes and soluble elements (e.g., Type I Interferons) influence LP CD4 T cell loss of life and performance.  We additionally describe the preparation of virus shares of Transmitted/Founder (TF) HIV-1 infectious molecular clones that have been efficiently utilized in the LPAC mannequin.

Co-culture techniques using reconstituted or artificial extracellular matrix (ECM) and micropatterning methods have enabled the reconstruction of floor epithelial tissues. This method has been utilized in the regeneration, illness modeling and drug screening of the floor epithelia, corresponding to the pores and skin and esophagus. On the different hand, the reconstruction of glandular epithelia would require extra intricate ECM organizations. Here we describe a protocol for a novel three-dimensional organotypic co-culture system for the reconstruction of mammary glands that makes use of the discontinuous ECM. In this system, main mammary fibroblasts first set up a layer of the connective tissue wealthy in collagen I.

Then, mammary epithelial cells type acinar buildings, the purposeful glandular items, inside the laminin-rich basement membrane embedded in the connective tissue. This methodology permits for the regeneration of the in vivo-like structure of mammary glands and could possibly be utilized for monitoring the real-time response of mammary glands to drug remedy. Here, we element the protocol to ascertain LP CD4 T cell an infection utilizing a strategy of spinoculation, the subsequent analysis of an infection ranges utilizing multicolor circulate cytometry and the willpower of general LP CD4 T cell loss of life utilizing absolute LP CD4 T cell counts.

Using Imaging Flow Cytometry to Characterize Extracellular Vesicles Isolated from Cell Culture Media, Plasma or Urine

 

The capability to non-invasively detect particular injury to the kidney has been restricted. Identification of extracellular vesicles launched by cells, particularly when below duress, may permit for monitoring and identification of particular cell sorts inside the kidney which can be confused. We have tailored a beforehand revealed conventional circulate cytometry methodology to be used with an imaging circulate cytometer (Amnis FlowSight) for figuring out EV launched by particular cell sorts and excreted into the urine or blood utilizing markers attribute of explicit cells in the kidney. Here we current a protocol using the Amnis FlowSight Imaging Flow Cytometer to determine and quantify EV from the urine of sufferers with important hypertension and renovascular illness. Notably, EV remoted from cell tradition media and plasma may also be analyzed equally.

Dissolved oxygen and its availability to cells in tradition is an missed variable which may have vital penalties on experimental analysis outcomes, together with reproducibility. Oxygen sensing pathways play key roles in cell progress and habits and pericellular oxygen ranges ought to be managed when establishing in vitro fashions. Standard cell tradition methods wouldn’t have enough management over pericellular oxygen ranges. Slow diffusion by tradition media limits the precision of oxygen supply to cells, making it troublesome to precisely reproduce in vivo-like oxygen circumstances. Furthermore, several types of cells eat oxygen at various charges and this may be affected by the density of rising cells.

Here, we describe a novel in vitro system that makes use of hypoxic chambers and oxygen-permeable tradition dishes to regulate pericellular oxygen ranges and supply speedy oxygen supply to adherent cells. This process is especially related for protocols learning results of speedy oxygen modifications or intermittent hypoxia on mobile habits. The system is cheap and simply assembled with out extremely specialised tools. Single myofiber tradition has a number of benefits that the conventional strategy corresponding to FASC and cryosection couldn’t compete with.

Quantifying HIV-1-Mediated Gut CD4 + T Cell Deathin the Lamina Propria Aggregate Culture (LPAC) Model

Isolation and Culture of Single Myofiber and Immunostaining of Satellite Cells from Adult C57BL/6J Mice

 

Myofiber isolation adopted with ex vivo tradition might recapitulate and visualize satellite tv for pc cells (SCs) activation, proliferation, and differentiation. This strategy could possibly be taken to know the physiology of satellite tv for pc cells and the molecular mechanism of regulatory elements, by way of the involvement of intrinsic elements over SCs quiescence, activation, proliferation and differentiation. For instance, myofiber isolation and tradition could possibly be used to watch SCs activation, proliferation and differentiation at a steady method inside their physiological “area of interest” surroundings whereas FACS or cryosection might solely seize single time-point upon exterior stimulation to activate satellite tv for pc cells by BaCl2, Cardiotoxin or ischemia.

Long-term Cell Tracer 580 (Green) sample

P710GS NULL
EUR 0

Long-term Cell Tracer 580 (Yellow) sample

P710YS NULL
EUR 0

FluoGreen Tracer

SF41000 100 ul
EUR 435

CFDA SE Cell Tracer Kit

K1023-1 1 kit
EUR 189
Description: The CFDA SE (carboxyfluorescein diacetate succinimidyl ester) Cell Tracer Kit is usually used in fluorescence analyses as a long-term tracer of cells. The Kit can be used for in vitro and in vivo labeling of cells to determine whether or not a cell is proliferating, especially.

JAK2 JH2 Tracer

HY-102055 50mg
EUR 4596

TAK-580

HY-15246 10mg
EUR 408

MemBriteâ„¢ Fix 568/580

30095 1KIT
EUR 644
Description: Minimum order quantity: 1 unit of 1KIT

PTPN11 (Ab-580) Antibody

CSB-PA961746-
EUR 335
  • Form: liquid
  • Buffer: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibo
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Description: A polyclonal antibody against PTPN11 (Ab-580). Recognizes PTPN11 (Ab-580) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200

PTPN11 (Ab-580) Antibody

CSB-PA961746-100ul 100ul
EUR 316
  • Form: liquid
  • Buffer: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibo
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Description: A polyclonal antibody against PTPN11 (Ab-580). Recognizes PTPN11 (Ab-580) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200

hsa-miR-580 Primers

MPH01878 150 ul / 10 uM
EUR 121

SHP-2 (Ab-580) Antibody

21320-100ul 100ul
EUR 252

SHP-2 (Ab-580) Antibody

21320-50ul 50ul
EUR 187

PYK2 (Phospho-Tyr579/580) Antibody

13230-100ul 100ul
EUR 252

PYK2 (Phospho-Tyr579/580) Antibody

13230-50ul 50ul
EUR 187

Phospho-PYK2 (Tyr579/580) Antibody

AF7383 200ul
EUR 376
Description: Phospho-PYK2 (Tyr579/580) Antibody detects endogenous levels of PYK2 only when phosphorylated at Tyr579/580.

anti-SHP-2 (Ab-580)

LF-PA20454 100 ul
EUR 334
Description: Rabbit polyclonal to SHP-2

hsa-miR-580 miRNA Inhibitor

MIH03164 2 x 5.0 nmol
EUR 176

hsa-miR-580 miRNA Antagomir

MNH03164 2 x 5.0 nmol
EUR 329

hsa-miR-580-3p Primers

MPH03331 150 ul / 10 uM
EUR 121

hsa-miR-580-5p Primers

MPH03332 150 ul / 10 uM
EUR 121

MemBriteâ„¢ Fix 568/580, Trial Size

30095-T 1KIT
EUR 222
Description: Minimum order quantity: 1 unit of 1KIT

DAX-J2™ Ratio 580/460

16310 1 mg
EUR 393
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Zinc Finger Protein 580 (ZNF580) Antibody

abx030431-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

Zinc Finger Protein 580 (ZNF580) Antibody

abx030431-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

Phospho-PYK2 (Tyr579/580) Blocking Peptide

AF7383-BP 1mg
EUR 195

PYK2 (Phospho-Tyr579/580) Conjugated Antibody

C13230 100ul
EUR 397

Recombinant human Zinc finger protein 580

P2142 100ug Ask for price
  • Uniprot ID: Q9UK33
  • Reconstitution: Metal affinity chromatography on Fn Super Capacity Column (Nickel)
Description: Recombinant protein for human Zinc finger protein 580

hsa-miR-580-3p miRNA Inhibitor

MIH03165 2 x 5.0 nmol
EUR 176

hsa-miR-580-5p miRNA Inhibitor

MIH03166 2 x 5.0 nmol
EUR 176

hsa-miR-580-3p miRNA Antagomir

MNH03165 2 x 5.0 nmol
EUR 329

hsa-miR-580-5p miRNA Antagomir

MNH03166 2 x 5.0 nmol
EUR 329

Long trebler phosphoramidite

A8151-250 250 mg
EUR 340
Description: Long trebler phosphoramidite is a branching reagent for oligonucleotide synthesis allowing to synthesize branched DNA structures using standard DNA synthesizer.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135 96 assays
EUR 821
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135-5 5 x 96 assays
EUR 3356
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140 96 assays
EUR 856
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140-5 5 x 96 assays
EUR 3483
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

293AD Cell Line

AD-100 1 vial
EUR 461
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

293AAV Cell Line

AAV-100 1 vial
EUR 508
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293LTV Cell Line

LTV-100 1 vial
EUR 508
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.

293RTV Cell Line

RV-100 1 vial
EUR 508
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.

Human B Cell Leukemia X long ELISA kit

E01B0575-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Human B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human B Cell Leukemia X long ELISA kit

E01B0575-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human B Cell Leukemia X long ELISA kit

E01B0575-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse B Cell Leukemia X long ELISA kit

E03B0575-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse B Cell Leukemia X long ELISA kit

E03B0575-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse B Cell Leukemia X long ELISA kit

E03B0575-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat B Cell Leukemia X long ELISA kit

E06B0575-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat B Cell Leukemia X long ELISA kit

E06B0575-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat B Cell Leukemia X long ELISA kit

E06B0575-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Goat B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit B Cell Leukemia X long ELISA kit

E04B0575-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit B Cell Leukemia X long ELISA kit

E04B0575-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit B Cell Leukemia X long ELISA kit

E04B0575-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rabbit B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat B Cell Leukemia X long ELISA kit

E02B0575-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat B Cell Leukemia X long ELISA kit

E02B0575-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat B Cell Leukemia X long ELISA kit

E02B0575-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rat B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig B Cell Leukemia X long ELISA kit

E07B0575-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Porcine B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig B Cell Leukemia X long ELISA kit

E07B0575-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Porcine B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig B Cell Leukemia X long ELISA kit

E07B0575-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Porcine B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog B Cell Leukemia X long ELISA kit

E08B0575-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Canine B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog B Cell Leukemia X long ELISA kit

E08B0575-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Canine B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog B Cell Leukemia X long ELISA kit

E08B0575-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Canine B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey B Cell Leukemia X long ELISA kit

E09B0575-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Monkey B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey B Cell Leukemia X long ELISA kit

E09B0575-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Monkey B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey B Cell Leukemia X long ELISA kit

E09B0575-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Monkey B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325 96 assays
EUR 856
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325-5 5 x 96 assays
EUR 3361
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric, Trial Size

CBA-135-T 24 assays
EUR 432
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

293/GFP Cell Line

AKR-200 1 vial
EUR 572
Description: 293/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

T47D/GFP Cell Line

AKR-208 1 vial
EUR 572
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line

AKR-209 1 vial
EUR 572
Description: A549/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

HeLa/GFP Cell Line

AKR-213 1 vial
EUR 572
Description: HeLa/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/GFP Cell Line

AKR-214 1 vial
EUR 572
Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/Cas9 Cell Line

AKR-5104 1 vial
EUR 572

293/Cas9 Cell Line

AKR-5110 1 vial
EUR 572

HeLa/Cas9 Cell Line

AKR-5111 1 vial
EUR 572

RSV (Strain Long) Protein

abx069909-1ml 1 ml
EUR 328
  • Shipped within 5-10 working days.

RSV (Long Strain) Protein

abx069910-1mg 1 mg
EUR 2082
  • Shipped within 5-10 working days.

RSV Long Strain Protein

abx060644-100ug 100 ug
EUR 968
  • Shipped within 5-10 working days.

DISC1-long-specific Antibody

abx232397-100ug 100 ug
EUR 509
  • Shipped within 5-12 working days.

CytoSelect 96-Well Cell Transformation Assay (Cell Recovery Compatible, Fluorometric), Trial Size

CBA-140-T 24 assays
EUR 456
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

Collagen-based Cell Contraction Assay

CBA-201 24 assays
EUR 485
Description: Cell Biolabs? Collagen-based Contraction Assay Kit provides a simple system to assess cell contractivity in vitro and screen cell contraction mediators. Each kit provides sufficient quantities to perform up to 24 assays in a 24-well plate. The kit can be also used in culturing cells in 3D collagen matrix.

CytoSelect MTT Cell Proliferation Assay

CBA-252 960 assays
EUR 409
Description: Cell Biolabs? CytoSelect MTT Cell Proliferation Assay provides a colorimetric format for measuring and monitoring cell proliferation.  The kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates or 192 assays in 24-well plates.  Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then detected with the proliferation reagent, which is converted in live cells from the yellow tetrazole MTT to the purple formazan form by a cellular reductase (Figure 1).  An increase in cell proliferation is accompanied by an increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.

SKOV-3/Luc Cell Line

AKR-232 1 vial
EUR 572
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

MCF-7/Luc Cell Line

AKR-234 1 vial
EUR 572
Description: MCF-7/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

OVCAR-5/RFP Cell Line

AKR-254 1 vial
EUR 572
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

Human Zinc finger protein 580, ZNF580 ELISA KIT

ELI-51274h 96 Tests
EUR 824

Mouse Zinc finger protein 580, Znf580 ELISA KIT

ELI-51275m 96 Tests
EUR 865

Human pre-microRNA Expression Construct Lenti-miR-580

PMIRH580PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

Recombinant Toxoplasma Gondii MIC4 Protein (aa 1-580)

VAng-0439Lsx-inquire inquire Ask for price
Description: Toxoplasma Gondii Micronemal protein 4, recombinant protein.

Recombinant Mycobacterium Avium mhpA Protein (aa 1-580)

VAng-Yyj2652-inquire inquire Ask for price
Description: Mycobacterium Avium (strain 104) 3- (3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase (mhpA), recombinant protein.

Recombinant Mycobacterium Avium mhpA Protein(aa 1-580)

VAng-Yyj1323-inquire inquire Ask for price
Description: Mycobacterium Avium (strain 104) 3- (3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase (mhpA), recombinant protein.

Recombinant Mycobacterium Paratuberculosis mhpA Protein (aa 1-580)

VAng-Yyj0966-inquire inquire Ask for price
Description: Mycobacterium Paratuberculosis 3- (3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase (mhpA), recombinant protein.

Recombinant Mycobacterium Marinum fadD28 Protein (aa 1-580)

VAng-Yyj4351-inquire inquire Ask for price
Description: Mycobacterium Marinum (strain ATCC BAA-535 / M) Long-chain-fatty-acid--AMP ligase FadD28 (fadD28), recombinant protein.

Recombinant Mycobacterium Abscessus proS Protein (aa 1-580)

VAng-Yyj5520-inquire inquire Ask for price
Description: Mycobacterium Abscessus (strain ATCC 19977 / DSM 44196) Proline--tRNA ligase (proS), recombinant protein.

Recombinant Pseudomonas Aeruginosa PA14_21660 Protein (aa 1-580)

VAng-Cr0847-1mgEcoli 1 mg (E. coli)
EUR 7468
Description: Pseudomonas Aeruginosa (strain UCBPP-PA14) UPF0060 membrane protein PA14_21660, recombinant protein.

Recombinant Pseudomonas Aeruginosa PA14_21660 Protein (aa 1-580)

VAng-Cr0847-500gEcoli 500 µg (E. coli)
EUR 5034
Description: Pseudomonas Aeruginosa (strain UCBPP-PA14) UPF0060 membrane protein PA14_21660, recombinant protein.

Recombinant Pseudomonas Aeruginosa PA14_21660 Protein (aa 1-580)

VAng-Cr0847-50gEcoli 50 µg (E. coli)
EUR 3420
Description: Pseudomonas Aeruginosa (strain UCBPP-PA14) UPF0060 membrane protein PA14_21660, recombinant protein.

Human MSLN Protein (Glu 296-Gly 580) [Fc]

VAng-2562Lsx-100g 100 µg
EUR 820
Description: Human Mesothelin protein, human IgG1 Fc tag, was expressed in human 293 cells. (Uniprot ID: AAH09272)

Furthermore, in vitro transfection with siRNA or overexpression vector could possibly be carried out below ex vivo tradition to know the detailed molecular operate of a selected gene on SCs physiology. With these benefits, the physiological state of SCs could possibly be analyzed at a number of designated time-points by immunofluorescence staining. In this protocol, we offer an environment friendly and sensible protocol to isolate single myofiber from EDL muscle, adopted with ex vivo tradition and immunostaining.