Quantifying HIV-1-Mediated Gut CD4 + T Cell Deathin the Lamina Propria Aggregate Culture (LPAC) Model

Gut CD4 T cells are main targets of HIV-1 and are massively depleted early throughout an infection. To higher perceive the mechanisms governing HIV-1-mediated CD4 T cell loss of life, we developed the physiologically-relevant Lamina Propria Aggregate Culture (LPAC) mannequin. The LPAC mannequin is right for learning CD4 T cell loss of life induced by clinically-relevant Transmitted/Founder (TF) HIV-1 strains and can also be appropriate for learning how enteric microbes and soluble elements (e.g., Type I Interferons) influence LP CD4 T cell loss of life and performance.  We additionally describe the preparation of virus shares of Transmitted/Founder (TF) HIV-1 infectious molecular clones that have been efficiently utilized in the LPAC mannequin.

Co-culture techniques using reconstituted or artificial extracellular matrix (ECM) and micropatterning methods have enabled the reconstruction of floor epithelial tissues. This method has been utilized in the regeneration, illness modeling and drug screening of the floor epithelia, corresponding to the pores and skin and esophagus. On the different hand, the reconstruction of glandular epithelia would require extra intricate ECM organizations. Here we describe a protocol for a novel three-dimensional organotypic co-culture system for the reconstruction of mammary glands that makes use of the discontinuous ECM. In this system, main mammary fibroblasts first set up a layer of the connective tissue wealthy in collagen I.

Then, mammary epithelial cells type acinar buildings, the purposeful glandular items, inside the laminin-rich basement membrane embedded in the connective tissue. This methodology permits for the regeneration of the in vivo-like structure of mammary glands and could possibly be utilized for monitoring the real-time response of mammary glands to drug remedy. Here, we element the protocol to ascertain LP CD4 T cell an infection utilizing a strategy of spinoculation, the subsequent analysis of an infection ranges utilizing multicolor circulate cytometry and the willpower of general LP CD4 T cell loss of life utilizing absolute LP CD4 T cell counts.

 

The capability to non-invasively detect particular injury to the kidney has been restricted. Identification of extracellular vesicles launched by cells, particularly when below duress, may permit for monitoring and identification of particular cell sorts inside the kidney which can be confused. We have tailored a beforehand revealed conventional circulate cytometry methodology to be used with an imaging circulate cytometer (Amnis FlowSight) for figuring out EV launched by particular cell sorts and excreted into the urine or blood utilizing markers attribute of explicit cells in the kidney. Here we current a protocol using the Amnis FlowSight Imaging Flow Cytometer to determine and quantify EV from the urine of sufferers with important hypertension and renovascular illness. Notably, EV remoted from cell tradition media and plasma may also be analyzed equally.

Dissolved oxygen and its availability to cells in tradition is an missed variable which may have vital penalties on experimental analysis outcomes, together with reproducibility. Oxygen sensing pathways play key roles in cell progress and habits and pericellular oxygen ranges ought to be managed when establishing in vitro fashions. Standard cell tradition methods wouldn’t have enough management over pericellular oxygen ranges. Slow diffusion by tradition media limits the precision of oxygen supply to cells, making it troublesome to precisely reproduce in vivo-like oxygen circumstances. Furthermore, several types of cells eat oxygen at various charges and this may be affected by the density of rising cells.

Here, we describe a novel in vitro system that makes use of hypoxic chambers and oxygen-permeable tradition dishes to regulate pericellular oxygen ranges and supply speedy oxygen supply to adherent cells. This process is especially related for protocols learning results of speedy oxygen modifications or intermittent hypoxia on mobile habits. The system is cheap and simply assembled with out extremely specialised tools. Single myofiber tradition has a number of benefits that the conventional strategy corresponding to FASC and cryosection couldn’t compete with.

 

Myofiber isolation adopted with ex vivo tradition might recapitulate and visualize satellite tv for pc cells (SCs) activation, proliferation, and differentiation. This strategy could possibly be taken to know the physiology of satellite tv for pc cells and the molecular mechanism of regulatory elements, by way of the involvement of intrinsic elements over SCs quiescence, activation, proliferation and differentiation. For instance, myofiber isolation and tradition could possibly be used to watch SCs activation, proliferation and differentiation at a steady method inside their physiological “area of interest” surroundings whereas FACS or cryosection might solely seize single time-point upon exterior stimulation to activate satellite tv for pc cells by BaCl₂, Cardiotoxin or ischemia.

FluoGreen Tracer
SF41000	Neuromics	100 ul	EUR 435

CFDA SE Cell Tracer Kit
K1023-1	ApexBio	1 kit	EUR 189
Description: The CFDA SE (carboxyfluorescein diacetate succinimidyl ester) Cell Tracer Kit is usually used in fluorescence analyses as a long-term tracer of cells. The Kit can be used for in vitro and in vivo labeling of cells to determine whether or not a cell is proliferating, especially.

JAK2 JH2 Tracer
HY-102055	MedChemExpress	50mg	EUR 4596

TAK-580
HY-15246	MedChemExpress	10mg	EUR 408

MemBriteâ„¢ Fix 568/580
30095	Biotium	1KIT	EUR 644
Description: Minimum order quantity: 1 unit of 1KIT

PTPN11 (Ab-580) Antibody
CSB-PA961746-	Cusabio		EUR 335
Description: A polyclonal antibody against PTPN11 (Ab-580). Recognizes PTPN11 (Ab-580) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200

PTPN11 (Ab-580) Antibody
CSB-PA961746-100ul	Cusabio	100ul	EUR 316
Description: A polyclonal antibody against PTPN11 (Ab-580). Recognizes PTPN11 (Ab-580) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200

hsa-miR-580 Primers
MPH01878	ABM	150 ul / 10 uM	EUR 121

PYK2 (Phospho-Tyr579/580) Antibody
13230-100ul	SAB	100ul	EUR 252

PYK2 (Phospho-Tyr579/580) Antibody
13230-50ul	SAB	50ul	EUR 187

SHP-2 (Ab-580) Antibody
21320-100ul	SAB	100ul	EUR 252

SHP-2 (Ab-580) Antibody
21320-50ul	SAB	50ul	EUR 187

Phospho-PYK2 (Tyr579/580) Antibody
AF7383	Affbiotech	200ul	EUR 376
Description: Phospho-PYK2 (Tyr579/580) Antibody detects endogenous levels of PYK2 only when phosphorylated at Tyr579/580.

anti-SHP-2 (Ab-580)
LF-PA20454	Abfrontier	100 ul	EUR 334
Description: Rabbit polyclonal to SHP-2

hsa-miR-580 miRNA Inhibitor
MIH03164	ABM	2 x 5.0 nmol	EUR 176

hsa-miR-580 miRNA Antagomir
MNH03164	ABM	2 x 5.0 nmol	EUR 329

hsa-miR-580-3p Primers
MPH03331	ABM	150 ul / 10 uM	EUR 121

hsa-miR-580-5p Primers
MPH03332	ABM	150 ul / 10 uM	EUR 121

Zinc Finger Protein 580 (ZNF580) Antibody
abx030431-400ul	Abbexa	400 ul	EUR 523

Zinc Finger Protein 580 (ZNF580) Antibody
abx030431-80l	Abbexa	80 µl	EUR 286

MemBriteâ„¢ Fix 568/580, Trial Size
30095-T	Biotium	1KIT	EUR 222
Description: Minimum order quantity: 1 unit of 1KIT

DAX-J2™ Ratio 580/460
16310	AAT Bioquest	1 mg	EUR 393

Recombinant human Zinc finger protein 580
P2142	FN Test	100ug	Ask for price
Description: Recombinant protein for human Zinc finger protein 580

PYK2 (Phospho-Tyr579/580) Conjugated Antibody
C13230	SAB	100ul	EUR 397

Phospho-PYK2 (Tyr579/580) Blocking Peptide
AF7383-BP	Affbiotech	1mg	EUR 195

hsa-miR-580-3p miRNA Inhibitor
MIH03165	ABM	2 x 5.0 nmol	EUR 176

hsa-miR-580-5p miRNA Inhibitor
MIH03166	ABM	2 x 5.0 nmol	EUR 176

hsa-miR-580-3p miRNA Antagomir
MNH03165	ABM	2 x 5.0 nmol	EUR 329

hsa-miR-580-5p miRNA Antagomir
MNH03166	ABM	2 x 5.0 nmol	EUR 329

Mesothelin / MSLN (296-580) Recombinant Protein
11-216	ProSci	0.1 mg	EUR 595.25
Description: Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.

Serpin A1c Recombinant Protein
91-580	ProSci	0.05 mg	EUR 684.5
Description: Alpha-1-antitrypsin 1-3(SERPIN A1) is a secreted protein and belongs to the serpin family. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. Mouse SERPIN A1 is a serine protease inhibitor whose targets include elastase,plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema orliver disease. Several transcript variants encoding the same protein have been found for this gene.

Kallikrein 7 Recombinant Protein
92-580	ProSci	0.05 mg	EUR 684.5
Description: Kallikrein7, also named as stratum corneum chymotryptic enzyme (SCCE), is a secreted protein of the Kallikrein-related peptidase (KLK) family. This family contains fifteen homologous secreted serine endopeptidases and plays a significant role in various physiological processes, including skin desquamation, semen liquefaction, neural plasticity, and body fluid homeostasis. In skin KLK5, KLK 7 and KLK14 are able to degrade corneodesmosomes, which leads to desquamation of skin surface cells. KLK activation is believed to be mediated through highly organized proteolytic cascades, regulated through a series of feedback loops, inhibitors, auto-degradation and internal cleavages. Studies have shown that one potential physiological activator for KLK7 is KLK5. Along with KLK14, these three kallikreins form a proteolytic cascade in the stratum corneum. KLK7 is primarily expressed in the skin but is also detected at relatively high levels in esophagus, heart, liver, central nervous system, kidney, pancreas, mammary and salivary glands.

Long trebler phosphoramidite
A8151-250	ApexBio	250 mg	EUR 340
Description: Long trebler phosphoramidite is a branching reagent for oligonucleotide synthesis allowing to synthesize branched DNA structures using standard DNA synthesizer.

Rat B Cell Leukemia X long ELISA kit
E02B0575-192T	BlueGene	192 tests	EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat B Cell Leukemia X long ELISA kit
E02B0575-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A competitive ELISA for quantitative measurement of Rat B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse B Cell Leukemia X long ELISA kit
E03B0575-192T	BlueGene	192 tests	EUR 1270
Description: A competitive ELISA for quantitative measurement of Mouse B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse B Cell Leukemia X long ELISA kit
E03B0575-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A competitive ELISA for quantitative measurement of Mouse B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse B Cell Leukemia X long ELISA kit
E03B0575-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A competitive ELISA for quantitative measurement of Mouse B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog B Cell Leukemia X long ELISA kit
E08B0575-192T	BlueGene	192 tests	EUR 1270
Description: A competitive ELISA for quantitative measurement of Canine B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog B Cell Leukemia X long ELISA kit
E08B0575-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A competitive ELISA for quantitative measurement of Canine B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog B Cell Leukemia X long ELISA kit
E08B0575-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A competitive ELISA for quantitative measurement of Canine B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey B Cell Leukemia X long ELISA kit
E09B0575-192T	BlueGene	192 tests	EUR 1270
Description: A competitive ELISA for quantitative measurement of Monkey B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey B Cell Leukemia X long ELISA kit
E09B0575-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A competitive ELISA for quantitative measurement of Monkey B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey B Cell Leukemia X long ELISA kit
E09B0575-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A competitive ELISA for quantitative measurement of Monkey B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit B Cell Leukemia X long ELISA kit
E04B0575-192T	BlueGene	192 tests	EUR 1270
Description: A competitive ELISA for quantitative measurement of Rabbit B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit B Cell Leukemia X long ELISA kit
E04B0575-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A competitive ELISA for quantitative measurement of Rabbit B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit B Cell Leukemia X long ELISA kit
E04B0575-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A competitive ELISA for quantitative measurement of Rabbit B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig B Cell Leukemia X long ELISA kit
E07B0575-192T	BlueGene	192 tests	EUR 1270
Description: A competitive ELISA for quantitative measurement of Porcine B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig B Cell Leukemia X long ELISA kit
E07B0575-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A competitive ELISA for quantitative measurement of Porcine B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig B Cell Leukemia X long ELISA kit
E07B0575-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A competitive ELISA for quantitative measurement of Porcine B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat B Cell Leukemia X long ELISA kit
E06B0575-192T	BlueGene	192 tests	EUR 1270
Description: A competitive ELISA for quantitative measurement of Goat B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat B Cell Leukemia X long ELISA kit
E06B0575-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A competitive ELISA for quantitative measurement of Goat B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat B Cell Leukemia X long ELISA kit
E06B0575-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A competitive ELISA for quantitative measurement of Goat B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat B Cell Leukemia X long ELISA kit
E02B0575-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A competitive ELISA for quantitative measurement of Rat B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human B Cell Leukemia X long ELISA kit
E01B0575-192T	BlueGene	192 tests	EUR 1270
Description: A competitive ELISA for quantitative measurement of Human B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human B Cell Leukemia X long ELISA kit
E01B0575-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A competitive ELISA for quantitative measurement of Human B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human B Cell Leukemia X long ELISA kit
E01B0575-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A competitive ELISA for quantitative measurement of Human B Cell Leukemia X long in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric
CBA-135	Cell Biolabs	96 assays	EUR 821
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric
CBA-135-5	Cell Biolabs	5 x 96 assays	EUR 3356
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric
CBA-140	Cell Biolabs	96 assays	EUR 856
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric
CBA-140-5	Cell Biolabs	5 x 96 assays	EUR 3483
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

293LTV Cell Line
LTV-100	Cell Biolabs	1 vial	EUR 508
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.

293AD Cell Line
AD-100	Cell Biolabs	1 vial	EUR 461
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

293AAV Cell Line
AAV-100	Cell Biolabs	1 vial	EUR 508
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293RTV Cell Line
RV-100	Cell Biolabs	1 vial	EUR 508
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)
CBA-325	Cell Biolabs	96 assays	EUR 856
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)
CBA-325-5	Cell Biolabs	5 x 96 assays	EUR 3361
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric, Trial Size
CBA-135-T	Cell Biolabs	24 assays	EUR 432
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

293/GFP Cell Line
AKR-200	Cell Biolabs	1 vial	EUR 572
Description: 293/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

T47D/GFP Cell Line
AKR-208	Cell Biolabs	1 vial	EUR 572
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line
AKR-209	Cell Biolabs	1 vial	EUR 572
Description: A549/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

HeLa/GFP Cell Line
AKR-213	Cell Biolabs	1 vial	EUR 572
Description: HeLa/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/GFP Cell Line
AKR-214	Cell Biolabs	1 vial	EUR 572
Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/Cas9 Cell Line
AKR-5104	Cell Biolabs	1 vial	EUR 572

293/Cas9 Cell Line
AKR-5110	Cell Biolabs	1 vial	EUR 572

HeLa/Cas9 Cell Line
AKR-5111	Cell Biolabs	1 vial	EUR 572

RSV Long Strain Protein
abx060644-100ug	Abbexa	100 ug	EUR 968

RSV (Strain Long) Protein
abx069909-1ml	Abbexa	1 ml	EUR 328

RSV (Long Strain) Protein
abx069910-1mg	Abbexa	1 mg	EUR 2082

DISC1-long-specific Antibody
abx232397-100ug	Abbexa	100 ug	EUR 509

Recombinant Toxoplasma Gondii MIC4 Protein (aa 1-580)
VAng-0439Lsx-inquire	Creative Biolabs	inquire	Ask for price
Description: Toxoplasma Gondii Micronemal protein 4, recombinant protein.

Human MSLN Protein (Glu 296-Gly 580) [Fc]
VAng-2562Lsx-100g	Creative Biolabs	100 µg	EUR 820
Description: Human Mesothelin protein, human IgG1 Fc tag, was expressed in human 293 cells. (Uniprot ID: AAH09272)

Human MSLN Protein (Glu 296-Gly 580) [Fc]
VAng-2562Lsx-1mg	Creative Biolabs	1 mg	EUR 4999
Description: Human Mesothelin protein, human IgG1 Fc tag, was expressed in human 293 cells. (Uniprot ID: AAH09272)

Human MSLN Protein (Glu 296-Gly 580) [His]
VAng-2563Lsx-100g	Creative Biolabs	100 µg	EUR 916
Description: Human Mesothelin protein, His tag, was expressed in human 293 cells. (Uniprot ID: AAH09272)

Human MSLN Protein (Glu 296-Gly 580) [His]
VAng-2563Lsx-1mg	Creative Biolabs	1 mg	EUR 5494
Description: Human Mesothelin protein, His tag, was expressed in human 293 cells. (Uniprot ID: AAH09272)

Recombinant Pseudomonas Aeruginosa PA14_21660 Protein (aa 1-580)
VAng-Cr0847-1mgEcoli	Creative Biolabs	1 mg (E. coli)	EUR 7468
Description: Pseudomonas Aeruginosa (strain UCBPP-PA14) UPF0060 membrane protein PA14_21660, recombinant protein.

Recombinant Pseudomonas Aeruginosa PA14_21660 Protein (aa 1-580)
VAng-Cr0847-500gEcoli	Creative Biolabs	500 µg (E. coli)	EUR 5034
Description: Pseudomonas Aeruginosa (strain UCBPP-PA14) UPF0060 membrane protein PA14_21660, recombinant protein.

Recombinant Pseudomonas Aeruginosa PA14_21660 Protein (aa 1-580)
VAng-Cr0847-50gEcoli	Creative Biolabs	50 µg (E. coli)	EUR 3420
Description: Pseudomonas Aeruginosa (strain UCBPP-PA14) UPF0060 membrane protein PA14_21660, recombinant protein.

Recombinant Mycobacterium Paratuberculosis mhpA Protein (aa 1-580)
VAng-Yyj0966-inquire	Creative Biolabs	inquire	Ask for price
Description: Mycobacterium Paratuberculosis 3- (3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase (mhpA), recombinant protein.

Recombinant Mycobacterium Avium mhpA Protein（aa 1-580)
VAng-Yyj1323-inquire	Creative Biolabs	inquire	Ask for price
Description: Mycobacterium Avium (strain 104) 3- (3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase (mhpA), recombinant protein.

Recombinant Mycobacterium Avium mhpA Protein (aa 1-580)
VAng-Yyj2652-inquire	Creative Biolabs	inquire	Ask for price
Description: Mycobacterium Avium (strain 104) 3- (3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase (mhpA), recombinant protein.

Recombinant Brucella Abortus fliF Protein (aa 1-580)
VAng-Lsx4789-inquire	Creative Biolabs	inquire	Ask for price
Description: Brucella Abortus biovar 1 (strain 9-941) Flagellar M-ring protein, recombinant protein.

Recombinant Mycobacterium Marinum fadD28 Protein (aa 1-580)
VAng-Yyj4351-inquire	Creative Biolabs	inquire	Ask for price
Description: Mycobacterium Marinum (strain ATCC BAA-535 / M) Long-chain-fatty-acid--AMP ligase FadD28 (fadD28), recombinant protein.

Recombinant Mycobacterium Abscessus proS Protein (aa 1-580)
VAng-Yyj5520-inquire	Creative Biolabs	inquire	Ask for price
Description: Mycobacterium Abscessus (strain ATCC 19977 / DSM 44196) Proline--tRNA ligase (proS), recombinant protein.

Human Zinc finger protein 580, ZNF580 ELISA KIT
ELI-51274h	Lifescience Market	96 Tests	EUR 824

Mouse Zinc finger protein 580, Znf580 ELISA KIT
ELI-51275m	Lifescience Market	96 Tests	EUR 865

Furthermore, in vitro transfection with siRNA or overexpression vector could possibly be carried out below ex vivo tradition to know the detailed molecular operate of a selected gene on SCs physiology. With these benefits, the physiological state of SCs could possibly be analyzed at a number of designated time-points by immunofluorescence staining. In this protocol, we offer an environment friendly and sensible protocol to isolate single myofiber from EDL muscle, adopted with ex vivo tradition and immunostaining.