Gut CD4 T cells are main targets of HIV-1 and are massively depleted early throughout an infection. To higher perceive the mechanisms governing HIV-1-mediated CD4 T cell loss of life, we developed the physiologically-relevant Lamina Propria Aggregate Culture (LPAC) mannequin. The LPAC mannequin is right for learning CD4 T cell loss of life induced by clinically-relevant Transmitted/Founder (TF) HIV-1 strains and can also be appropriate for learning how enteric microbes and soluble elements (e.g., Type I Interferons) influence LP CD4 T cell loss of life and performance. We additionally describe the preparation of virus shares of Transmitted/Founder (TF) HIV-1 infectious molecular clones that have been efficiently utilized in the LPAC mannequin.
Co-culture techniques using reconstituted or artificial extracellular matrix (ECM) and micropatterning methods have enabled the reconstruction of floor epithelial tissues. This method has been utilized in the regeneration, illness modeling and drug screening of the floor epithelia, corresponding to the pores and skin and esophagus. On the different hand, the reconstruction of glandular epithelia would require extra intricate ECM organizations. Here we describe a protocol for a novel three-dimensional organotypic co-culture system for the reconstruction of mammary glands that makes use of the discontinuous ECM. In this system, main mammary fibroblasts first set up a layer of the connective tissue wealthy in collagen I.
Then, mammary epithelial cells type acinar buildings, the purposeful glandular items, inside the laminin-rich basement membrane embedded in the connective tissue. This methodology permits for the regeneration of the in vivo-like structure of mammary glands and could possibly be utilized for monitoring the real-time response of mammary glands to drug remedy. Here, we element the protocol to ascertain LP CD4 T cell an infection utilizing a strategy of spinoculation, the subsequent analysis of an infection ranges utilizing multicolor circulate cytometry and the willpower of general LP CD4 T cell loss of life utilizing absolute LP CD4 T cell counts.
Using Imaging Flow Cytometry to Characterize Extracellular Vesicles Isolated from Cell Culture Media, Plasma or Urine
The capability to non-invasively detect particular injury to the kidney has been restricted. Identification of extracellular vesicles launched by cells, particularly when below duress, may permit for monitoring and identification of particular cell sorts inside the kidney which can be confused. We have tailored a beforehand revealed conventional circulate cytometry methodology to be used with an imaging circulate cytometer (Amnis FlowSight) for figuring out EV launched by particular cell sorts and excreted into the urine or blood utilizing markers attribute of explicit cells in the kidney. Here we current a protocol using the Amnis FlowSight Imaging Flow Cytometer to determine and quantify EV from the urine of sufferers with important hypertension and renovascular illness. Notably, EV remoted from cell tradition media and plasma may also be analyzed equally.
Dissolved oxygen and its availability to cells in tradition is an missed variable which may have vital penalties on experimental analysis outcomes, together with reproducibility. Oxygen sensing pathways play key roles in cell progress and habits and pericellular oxygen ranges ought to be managed when establishing in vitro fashions. Standard cell tradition methods wouldn’t have enough management over pericellular oxygen ranges. Slow diffusion by tradition media limits the precision of oxygen supply to cells, making it troublesome to precisely reproduce in vivo-like oxygen circumstances. Furthermore, several types of cells eat oxygen at various charges and this may be affected by the density of rising cells.
Here, we describe a novel in vitro system that makes use of hypoxic chambers and oxygen-permeable tradition dishes to regulate pericellular oxygen ranges and supply speedy oxygen supply to adherent cells. This process is especially related for protocols learning results of speedy oxygen modifications or intermittent hypoxia on mobile habits. The system is cheap and simply assembled with out extremely specialised tools. Single myofiber tradition has a number of benefits that the conventional strategy corresponding to FASC and cryosection couldn’t compete with.
Isolation and Culture of Single Myofiber and Immunostaining of Satellite Cells from Adult C57BL/6J Mice
Myofiber isolation adopted with ex vivo tradition might recapitulate and visualize satellite tv for pc cells (SCs) activation, proliferation, and differentiation. This strategy could possibly be taken to know the physiology of satellite tv for pc cells and the molecular mechanism of regulatory elements, by way of the involvement of intrinsic elements over SCs quiescence, activation, proliferation and differentiation. For instance, myofiber isolation and tradition could possibly be used to watch SCs activation, proliferation and differentiation at a steady method inside their physiological “area of interest” surroundings whereas FACS or cryosection might solely seize single time-point upon exterior stimulation to activate satellite tv for pc cells by BaCl2, Cardiotoxin or ischemia.
Long-term Cell Tracer 580 (Green) sample
P710GS
101Bio
NULL
EUR 0
Long-term Cell Tracer 580 (Yellow) sample
P710YS
101Bio
NULL
EUR 0
FluoGreen Tracer
SF41000
Neuromics
100 ul
EUR 435
CFDA SE Cell Tracer Kit
K1023-1
ApexBio
1 kit
EUR 189
JAK2 JH2 Tracer
HY-102055
MedChemExpress
50mg
EUR 4596
TAK-580
HY-15246
MedChemExpress
10mg
EUR 408
MemBriteâ„¢ Fix 568/580
30095
Biotium
1KIT
EUR 644
PTPN11 (Ab-580) Antibody
CSB-PA961746-
Cusabio
EUR 335
PTPN11 (Ab-580) Antibody
CSB-PA961746-100ul
Cusabio
100ul
EUR 316
hsa-miR-580 Primers
MPH01878
ABM
150 ul / 10 uM
EUR 121
Phospho-PYK2 (Tyr579/580) Antibody
AF7383
Affbiotech
200ul
EUR 376
PYK2 (Phospho-Tyr579/580) Antibody
13230-100ul
SAB
100ul
EUR 252
PYK2 (Phospho-Tyr579/580) Antibody
13230-50ul
SAB
50ul
EUR 187
SHP-2 (Ab-580) Antibody
21320-100ul
SAB
100ul
EUR 252
SHP-2 (Ab-580) Antibody
21320-50ul
SAB
50ul
EUR 187
hsa-miR-580-3p Primers
MPH03331
ABM
150 ul / 10 uM
EUR 121
hsa-miR-580-5p Primers
MPH03332
ABM
150 ul / 10 uM
EUR 121
hsa-miR-580 miRNA Inhibitor
MIH03164
ABM
2 x 5.0 nmol
EUR 176
hsa-miR-580 miRNA Antagomir
MNH03164
ABM
2 x 5.0 nmol
EUR 329
anti-SHP-2 (Ab-580)
LF-PA20454
Abfrontier
100 ul
EUR 334
PYK2 (Phospho-Tyr579/580) Conjugated Antibody
C13230
SAB
100ul
EUR 397
Phospho-PYK2 (Tyr579/580) Blocking Peptide
AF7383-BP
Affbiotech
1mg
EUR 195
Zinc Finger Protein 580 (ZNF580) Antibody
abx030431-400ul
Abbexa
400 ul
EUR 523
Zinc Finger Protein 580 (ZNF580) Antibody
abx030431-80l
Abbexa
80 µl
EUR 286
DAX-J2™ Ratio 580/460
16310
AAT Bioquest
1 mg
EUR 393
MemBriteâ„¢ Fix 568/580, Trial Size
30095-T
Biotium
1KIT
EUR 222
hsa-miR-580-3p miRNA Inhibitor
MIH03165
ABM
2 x 5.0 nmol
EUR 176
hsa-miR-580-5p miRNA Inhibitor
MIH03166
ABM
2 x 5.0 nmol
EUR 176
hsa-miR-580-3p miRNA Antagomir
MNH03165
ABM
2 x 5.0 nmol
EUR 329
hsa-miR-580-5p miRNA Antagomir
MNH03166
ABM
2 x 5.0 nmol
EUR 329
Recombinant human Zinc finger protein 580
P2142
FN Test
100ug
Ask for price
Long trebler phosphoramidite
A8151-250
ApexBio
250 mg
EUR 340
CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric
CBA-135
Cell Biolabs
96 assays
EUR 821
CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric
CBA-135-5
Cell Biolabs
5 x 96 assays
EUR 3356
CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric
CBA-140
Cell Biolabs
96 assays
EUR 856
CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric
CBA-140-5
Cell Biolabs
5 x 96 assays
EUR 3483
293AD Cell Line
AD-100
Cell Biolabs
1 vial
EUR 461
293AAV Cell Line
AAV-100
Cell Biolabs
1 vial
EUR 508
293LTV Cell Line
LTV-100
Cell Biolabs
1 vial
EUR 508
293RTV Cell Line
RV-100
Cell Biolabs
1 vial
EUR 508
Goat B Cell Leukemia X long ELISA kit
E06B0575-192T
B-Gene
192 tests
EUR 1270
Goat B Cell Leukemia X long ELISA kit
E06B0575-48
B-Gene
1 plate of 48 wells
EUR 520
Goat B Cell Leukemia X long ELISA kit
E06B0575-96
B-Gene
1 plate of 96 wells
EUR 685
Human B Cell Leukemia X long ELISA kit
E01B0575-192T
B-Gene
192 tests
EUR 1270
Human B Cell Leukemia X long ELISA kit
E01B0575-48
B-Gene
1 plate of 48 wells
EUR 520
Human B Cell Leukemia X long ELISA kit
E01B0575-96
B-Gene
1 plate of 96 wells
EUR 685
Mouse B Cell Leukemia X long ELISA kit
E03B0575-192T
B-Gene
192 tests
EUR 1270
Mouse B Cell Leukemia X long ELISA kit
E03B0575-48
B-Gene
1 plate of 48 wells
EUR 520
Mouse B Cell Leukemia X long ELISA kit
E03B0575-96
B-Gene
1 plate of 96 wells
EUR 685
Rabbit B Cell Leukemia X long ELISA kit
E04B0575-192T
B-Gene
192 tests
EUR 1270
Rabbit B Cell Leukemia X long ELISA kit
E04B0575-48
B-Gene
1 plate of 48 wells
EUR 520
Rabbit B Cell Leukemia X long ELISA kit
E04B0575-96
B-Gene
1 plate of 96 wells
EUR 685
Rat B Cell Leukemia X long ELISA kit
E02B0575-192T
B-Gene
192 tests
EUR 1270
Rat B Cell Leukemia X long ELISA kit
E02B0575-48
B-Gene
1 plate of 48 wells
EUR 520
Rat B Cell Leukemia X long ELISA kit
E02B0575-96
B-Gene
1 plate of 96 wells
EUR 685
Pig B Cell Leukemia X long ELISA kit
E07B0575-192T
B-Gene
192 tests
EUR 1270
Pig B Cell Leukemia X long ELISA kit
E07B0575-48
B-Gene
1 plate of 48 wells
EUR 520
Pig B Cell Leukemia X long ELISA kit
E07B0575-96
B-Gene
1 plate of 96 wells
EUR 685
Monkey B Cell Leukemia X long ELISA kit
E09B0575-192T
B-Gene
192 tests
EUR 1270
Monkey B Cell Leukemia X long ELISA kit
E09B0575-48
B-Gene
1 plate of 48 wells
EUR 520
Monkey B Cell Leukemia X long ELISA kit
E09B0575-96
B-Gene
1 plate of 96 wells
EUR 685
Dog B Cell Leukemia X long ELISA kit
E08B0575-192T
B-Gene
192 tests
EUR 1270
Dog B Cell Leukemia X long ELISA kit
E08B0575-48
B-Gene
1 plate of 48 wells
EUR 520
Dog B Cell Leukemia X long ELISA kit
E08B0575-96
B-Gene
1 plate of 96 wells
EUR 685
StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)
CBA-325
Cell Biolabs
96 assays
EUR 856
StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)
CBA-325-5
Cell Biolabs
5 x 96 assays
EUR 3361
CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric, Trial Size
CBA-135-T
Cell Biolabs
24 assays
EUR 432
293/GFP Cell Line
AKR-200
Cell Biolabs
1 vial
EUR 572
T47D/GFP Cell Line
AKR-208
Cell Biolabs
1 vial
EUR 572
A549/GFP Cell Line
AKR-209
Cell Biolabs
1 vial
EUR 572
HeLa/GFP Cell Line
AKR-213
Cell Biolabs
1 vial
EUR 572
NIH3T3/GFP Cell Line
AKR-214
Cell Biolabs
1 vial
EUR 572
NIH3T3/Cas9 Cell Line
AKR-5104
Cell Biolabs
1 vial
EUR 572
293/Cas9 Cell Line
AKR-5110
Cell Biolabs
1 vial
EUR 572
HeLa/Cas9 Cell Line
AKR-5111
Cell Biolabs
1 vial
EUR 572
RSV (Strain Long) Protein
abx069909-1ml
Abbexa
1 ml
EUR 328
RSV (Long Strain) Protein
abx069910-1mg
Abbexa
1 mg
EUR 2082
RSV Long Strain Protein
abx060644-100ug
Abbexa
100 ug
EUR 968
DISC1-long-specific Antibody
abx232397-100ug
Abbexa
100 ug
EUR 509
CytoSelect 96-Well Cell Transformation Assay (Cell Recovery Compatible, Fluorometric), Trial Size
CBA-140-T
Cell Biolabs
24 assays
EUR 456
SKOV-3/Luc Cell Line
AKR-232
Cell Biolabs
1 vial
EUR 572
MCF-7/Luc Cell Line
AKR-234
Cell Biolabs
1 vial
EUR 572
OVCAR-5/RFP Cell Line
AKR-254
Cell Biolabs
1 vial
EUR 572
Collagen-based Cell Contraction Assay
CBA-201
Cell Biolabs
24 assays
EUR 485
CytoSelect MTT Cell Proliferation Assay
CBA-252
Cell Biolabs
960 assays
EUR 409
Human Zinc finger protein 580, ZNF580 ELISA KIT
ELI-51274h
Lifescience Market
96 Tests
EUR 824
Mouse Zinc finger protein 580, Znf580 ELISA KIT
ELI-51275m
Lifescience Market
96 Tests
EUR 865
Human pre-microRNA Expression Construct Lenti-miR-580
PMIRH580PA-1
SBI
Bacterial Streak
EUR 684
Recombinant Toxoplasma Gondii MIC4 Protein (aa 1-580)
VAng-0439Lsx-inquire
Creative Biolabs
inquire
Ask for price
Human MSLN Protein (Glu 296-Gly 580) [Fc]
VAng-2562Lsx-100g
Creative Biolabs
100 µg
EUR 820
Human MSLN Protein (Glu 296-Gly 580) [Fc]
VAng-2562Lsx-1mg
Creative Biolabs
1 mg
EUR 4999
Human MSLN Protein (Glu 296-Gly 580) [His]
VAng-2563Lsx-100g
Creative Biolabs
100 µg
EUR 916
Human MSLN Protein (Glu 296-Gly 580) [His]
VAng-2563Lsx-1mg
Creative Biolabs
1 mg
EUR 5494
Recombinant Mycobacterium Paratuberculosis mhpA Protein (aa 1-580)
VAng-Yyj0966-inquire
Creative Biolabs
inquire
Ask for price
Recombinant Pseudomonas Aeruginosa PA14_21660 Protein (aa 1-580)
VAng-Cr0847-1mgEcoli
Creative Biolabs
1 mg (E. coli)
EUR 7468
Recombinant Pseudomonas Aeruginosa PA14_21660 Protein (aa 1-580)
VAng-Cr0847-500gEcoli
Creative Biolabs
500 µg (E. coli)
EUR 5034
Recombinant Pseudomonas Aeruginosa PA14_21660 Protein (aa 1-580)
VAng-Cr0847-50gEcoli
Creative Biolabs
50 µg (E. coli)
EUR 3420
Recombinant Mycobacterium Avium mhpA Protein(aa 1-580)
VAng-Yyj1323-inquire
Creative Biolabs
inquire
Ask for price
Recombinant Mycobacterium Avium mhpA Protein (aa 1-580)
VAng-Yyj2652-inquire
Creative Biolabs
inquire
Ask for price
Recombinant Brucella Abortus fliF Protein (aa 1-580)
VAng-Lsx4789-inquire
Creative Biolabs
inquire
Ask for price
Recombinant Mycobacterium Abscessus proS Protein (aa 1-580)
VAng-Yyj5520-inquire
Creative Biolabs
inquire
Ask for price
Recombinant Mycobacterium Marinum fadD28 Protein (aa 1-580)
VAng-Yyj4351-inquire
Creative Biolabs
inquire
Ask for price
Guinea pig B Cell Leukemia X long ELISA kit
E05B0575-192T
B-Gene
192 tests
EUR 1270
Guinea pig B Cell Leukemia X long ELISA kit
E05B0575-48
B-Gene
1 plate of 48 wells
EUR 520
Guinea pig B Cell Leukemia X long ELISA kit
E05B0575-96
B-Gene
1 plate of 96 wells
EUR 685
CytoSelect 24-well Cell Invasion, Fluorometric
CBA-111
Cell Biolabs
12 assays
EUR 595
CytoSelect 96-well Cell Invasion, Fluorometric
CBA-112
Cell Biolabs
96 assays
EUR 757
Radius 24-Well Cell Migration Assay
CBA-125
Cell Biolabs
24 assays
EUR 502
Radius 24-Well Cell Migration Assay
CBA-125-5
Cell Biolabs
5 x 24 assays
EUR 1969
Radius 96-Well Cell Migration Assay
CBA-126
Cell Biolabs
96 assays
EUR 572
Radius 96-Well Cell Migration Assay
CBA-126-5
Cell Biolabs
5 x 96 assays
EUR 2248
Radius 384-Well Cell Migration Assay
CBA-127
Cell Biolabs
384 assays
EUR 601
Radius 384-Well Cell Migration Assay
CBA-127-5
Cell Biolabs
5 x 384 wells
EUR 2335
CytoSelect 96-well Cell Transformation Assay
CBA-130
Cell Biolabs
96 assays
EUR 722
CytoSelect 96-well Cell Transformation Assay
CBA-130-5
Cell Biolabs
5 x 96 assays
EUR 2886
CytoSelect Cell Viability and Cytotoxicity Assay
CBA-240
Cell Biolabs
96 assays
EUR 392
CytoSelect Cell Proliferation Assay Reagent
(Fluorometric)
CBA-250
Cell Biolabs
10 mL
EUR 409
CytoSelect BrdU Cell Proliferation ELISA Kit
CBA-251
Cell Biolabs
96 assays
EUR 531
CytoSelect Cell Proliferation Assay Reagent (Colorimetric)
CBA-253
Cell Biolabs
10 mL
EUR 409
Radius 48-Well Cell Migration Assay
CBA-5037
Cell Biolabs
48 assays
EUR 519
Radius 48-Well Cell Migration Assay
CBA-5037-5
Cell Biolabs
5 x 48 assays
EUR 2045
CytoSelect 24-well Cell Invasion Assay, Colorimetric
CBA-110
Cell Biolabs
12 assays
EUR 595
Furthermore, in vitro transfection with siRNA or overexpression vector could possibly be carried out below ex vivo tradition to know the detailed molecular operate of a selected gene on SCs physiology. With these benefits, the physiological state of SCs could possibly be analyzed at a number of designated time-points by immunofluorescence staining. In this protocol, we offer an environment friendly and sensible protocol to isolate single myofiber from EDL muscle, adopted with ex vivo tradition and immunostaining.