Mesenchymal stem cells have the flexibility to distinguish into a number of lineages, together with adipocytes, osteoblasts and chondrocytes. Mesenchymal stem cells could be induced to distinguish into chondrocytes in extracellular matrices, akin to alginate or collagen gel. Mesenchymal stem cells in a cell pellet or micromass tradition could be additionally induced to type cartilages in an outlined medium containing chondrogenic cytokines, akin to reworking development factor-β (TGF-β). Here, we describe a easy methodology to type cartilage by seeding mesenchymal cells derived from limb-bud cells at excessive cell density.
First, we dissected the limb buds from embryonic mice (embryonic day 12.5) and digested them with enzymes (dispase and collagenase). After filtration utilizing a cell strainer, we seeded the cells at excessive density. Unlike different strategies, the strategy described right here is straightforward and doesn’t require using specialised tools, costly supplies or complicated reagents. The use of mouse and human cells, for instance, in one system permits for species-specific FACS or quantitative PCR evaluation. This protocol describes using a 3D Co-culture System of Mouse Prostatic Wild-type Fibroblasts with Human Prostate Cancer Epithelial Cells.
Heterogeneous prostatic carcinoma-associated fibroblasts (CAF) contribute to tumor development. This was established utilizing transgenic mouse fashions. Paracrine interactions between fibroblasts and epithelial cells had been additional interrogated utilizing remoted 2D cell tradition methods, however 3D tradition methods presently being developed can higher mimic reciprocal interactions probably discovered in the native tissue. To perceive paracrine and juxtacrine signaling amongst fibroblasts and epithelia, 3D co-cultures with species variations permits for additional subsequent evaluation of the cultures.
Co-tradition with Tetragenococcus halophilus improved the ethanol tolerance of Zygosaccharomyces rouxii by sustaining cell floor properties
The accumulation of ethanol has a unfavourable impact on the viability and fermentation efficiency of microorganisms through the manufacturing of fermented meals due to its toxicity. In this research, we investigated the impact of co-culture with Tetragenococcus halophilus on ethanol stress resistance of Zygosaccharomyces rouxii. The outcome confirmed that co-culture with T. halophilus promoted cell survival of Z. rouxii below ethanol stress, and the tolerance improved with growing co-culture time when ethanol content material was 8%. Physiological evaluation confirmed that the co-cultured Z. rouxii cells maintained greater intracellular content material of trehalose and amino acids together with tyrosine, tryptophan, arginine and proline after 8% ethanol stress for 90 min. The membrane integrity evaluation and biophysical evaluation of the cell floor indicated that the presence of ethanol resulted in cell membrane harm and adjustments of Young’s modulus worth and roughness of cell floor.
While the co-cultured Z. rouxii cells exhibited higher membrane integrity, stiffer and smoother cell floor than single-cultured cells below ethanol stress. As for transcriptomic analyses, the genes concerned in unsaturated fatty acid biosynthesis, trehalose biosynthesis, varied sorts of N-glycan biosynthesis, inositol phosphate metabolism, MAPK signaling pathway and tight junction had greater expression in co-cultured Z. rouxii cells with down-regulation of majority of gene expression after stress. And these genes might operate in the development of ethanol tolerance of Z. rouxii in co-culture. H460 cells had been cultured to the 10th technology by including a combination of spirulina animal cell tradition resolution (SACCS) and FBS to the tradition medium.
Field-based analysis on irritation and well being is often restricted to baseline measures of circulating cytokines or acute section proteins, whereas lab-based research can pursue a extra dynamic strategy with ex vivo cell tradition strategies. The laboratory infrastructure required for culturing leukocytes limits utility in community-based settings, which in flip limits scientific understandings of how psychosocial, behavioral, and contextual components affect the regulation of irritation. We goal to deal with this hole by validating two “field-friendly” cell tradition protocols, one utilizing a small quantity of venous complete blood, and one other utilizing finger stick capillary complete blood.
Marine cyanobacterium Spirulina maxima as an alternate to the animal cell tradition medium complement
Serum is a secure medium complement for in vitro cell tradition. Live cells are used in stem cell analysis, drug toxicity and security testing, illness analysis and prevention, and growth of antibiotics, medication, and vaccines. However, use of serum in tradition includes issues akin to an moral debate relating to the gathering course of, lack of standardizedsubstances, and excessivevalue. Herein, due to this fact, we evaluated the potential forutilizing edible cyanobacterium (Spirulina maxima), which is a nutrient-rich, sustainable, and ethically acceptable supply, as a novel substitute for fetal bovine serum (FBS).
 DiscoveryProbe? Stem Cell Compound Library |
|
L1040-.1 |
ApexBio |
100 uL/well(10 mM solution) |
EUR 4353 |
 Stem Cell MicroRNA Profiling Panel |
|
RA620A-1 |
SBI |
10 profiles |
EUR 1172 |
|
|
 AssayMax ELISA Kit) Mouse Stem Cell Factor (SCF) AssayMax ELISA Kit |
|
EMS1001-1 |
AssayPro |
96 Well Plate |
EUR 396 |
) Mouse Nanog Stem Cell Differentiation Reporter (pGreenZeo, plasmid) |
|
SR10031PA-1 |
SBI |
10 ug |
EUR 1749 |
|
|
) Mouse Nanog Stem Cell Differentiation Reporter (pGreenZeo, pre-packaged) |
|
SR10031VA-1 |
SBI |
>2 x 10^6 IFUs |
EUR 755 |
|
|
 Polyclonal Goat anti-GST α-form |
|
GST-ANTI-1 |
Detroit R&D |
50 uL |
EUR 280 |
 SCF Stem Cell Factor Human Recombinant Protein |
|
PROTP21583-1 |
BosterBio |
Regular: 10ug |
EUR 317 |
|
Description: Stem Cell Factor Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton. ;The SCF is purified by proprietary chromatographic techniques. |
) StemBoost? Neural Stem Cell Reprogramming Cocktail (100X) |
|
S238-1 |
Biovision |
|
EUR 185 |
 SCA-1 Antibody |
|
47996-100ul |
SAB |
100ul |
EUR 333 |
SCA-1 Antibody |
|
47996-50ul |
SAB |
50ul |
EUR 239 |
 AssayMax ELISA Kit) Human Stem Cell Factor (SCF) AssayMax ELISA Kit |
|
ES1001-1 |
AssayPro |
96 Well Plate |
EUR 396 |
 SCA-1 Conjugated Antibody |
|
C47996 |
SAB |
100ul |
EUR 397 |
) Mouse Prostate stem cell antigen (Psca) |
|
1-CSB-YP018840MO |
Cusabio |
-
EUR 504.00
-
EUR 265.00
-
EUR 1832.00
-
EUR 763.00
-
EUR 1216.00
-
EUR 334.00
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
|
|
Description: Recombinant Mouse Prostate stem cell antigen(Psca) expressed in Yeast |
 Anti-PARP-1 Antibody |
|
A00122-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal PARP-1 Antibody. Validated in ELISA, IP, IF, WB and tested in Human, Mouse. |
 Anti-Presenilin 1 Antibody |
|
A00138-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for Presenilin 1 Antibody (PSEN1) detection. Tested with WB, IHC in Human, Mouse, Rat. |
 Anti-CNG-1 Antibody |
|
A05494-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for CNG-1 Antibody (CNGA1) detection. Tested with WB in Human, Mouse, Rat. |
 Anti-Periphilin 1 Antibody |
|
A06996-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for Periphilin 1 Antibody (PPHLN1) detection.tested for WB in Human, Mouse. |
 Anti-Lyl-1 Antibody |
|
A07491-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Lyl-1 Antibody. Validated in IHC and tested in Human, Mouse, Rat. |
 Anti-GLI-1 Antibody |
|
A07972-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for GLI-1 Antibody (ACSS1) detection. Tested with WB in Human, Mouse, Rat. |
 Anti-Cerebellin 1 Antibody |
|
A09176-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for Cerebellin 1 Antibody (CBLN1) detection. Tested with WB in Human, Mouse, Rat. |
 Anti-DOC-1 Antibody |
|
A09467-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for DOC-1 Antibody (CDK2AP1) detection. Tested with WB in Human, Mouse. |
 Anti-PAI-1 Antibody |
|
A00637-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for PAI-1 Antibody (SERPINE1) detection.tested for WB in Human, Mouse, Rat. |
 Anti-Flk-1 Antibody |
|
A00901-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for Flk-1 Antibody (KDR) detection.tested for WB in Human, Mouse. |
 Anti-EDG-1 Antibody |
|
A01502-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for EDG-1 Antibody (S1PR1) detection.tested for WB in Human, Mouse, Rat. |
 Anti-ROBO-1 Antibody |
|
A01530-1 |
BosterBio |
100ug |
EUR 455 |
|
Description: Rabbit Polyclonal ROBO-1 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat. |
 Anti-NPDC-1 Antibody |
|
A12846-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for NPDC-1 Antibody (NPDC1) detection. Tested with WB in Human, Mouse, Rat. |
 Anti-Bag-1 Antibody |
|
A02423-1 |
BosterBio |
50 ul |
EUR 397 |
|
Description: Rabbit Polyclonal Bag-1 Antibody. Validated in IP, IHC and tested in Bovine, Canine, Human, Mouse, Rat. |
 Anti-TUB 1 Antibody |
|
A02917-1 |
BosterBio |
100ug/vial |
EUR 294 |
 Anti-Dok-1 Antibody |
|
A03039-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for Dok-1 Antibody (DOK1) detection.tested for WB in Human, Mouse, Rat. |
 Anti-TFIIIB90-1 Antibody |
|
A03761-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for TFIIIB90-1 Antibody (BRF1) detection.tested for WB in Human, Mouse. |
 Anti-Atrophin-1 Antibody |
|
A03828-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for Atrophin-1 Antibody (ATN1) detection. Tested with WB in Human, Mouse, Rat. |
) Anti-HO-1 Monoclonal Antibody (HO-1-2) |
|
M00253-1 |
BosterBio |
1mg |
EUR 397 |
|
Description: Mouse Monoclonal HO-1 Antibody (HO-1-2). Validated in Flow Cytometry, IHC, WB and tested in Human, Mouse, Rat. |
 Anti-Presenilin 1 Monoclonal Antibody |
|
M00138-1 |
BosterBio |
100ug |
EUR 397 |
|
Description: Rabbit Monoclonal Presenilin 1 Antibody. Validated in WB and tested in Human, Mouse, Rat. |
 Anti-Galectin 1 Monoclonal Antibody |
|
M00470-1 |
BosterBio |
100ug |
EUR 397 |
|
Description: Rabbit Monoclonal Galectin 1 Antibody. Validated in IP, IF, WB and tested in Human, Mouse, Rat. |
 Anti-DJ-1 Monoclonal Antibody |
|
M00757-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Mouse Monoclonal DJ-1 Antibody. Validated in IHC, WB and tested in Bovine, Human. |
 Anti-Enolase 1 Monoclonal Antibody |
|
M01250-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Anti-Enolase 1 Monoclonal Antibody tested in WB, IF, ICC, IHC, reactive to Human, rat, mouse, cow, pig, horse |
 Anti-Dynamin 1 Monoclonal Antibody |
|
M02536-1 |
BosterBio |
100ug |
EUR 397 |
|
Description: Rabbit Monoclonal Dynamin 1 Antibody. Validated in IF, WB and tested in Human, Mouse, Rat. |
 Anti-Esrp-1 Monoclonal Antibody |
|
M06068-1 |
BosterBio |
100uL |
EUR 443 |
|
Description: Mouse Monoclonal Esrp-1 Antibody. Validated in WB and tested in Human. |
 Anti-Angiopoietin 1/ANGPT1 Antibody |
|
PA1333-1 |
BosterBio |
100ug/vial |
EUR 334 |
 Anti-Galectin 1/LGALS1 Antibody |
|
PB9240-1 |
BosterBio |
100ug/vial |
EUR 334 |
 Anti-TNF Receptor 1 Antibody |
|
A00294-1 |
BosterBio |
200ug |
EUR 522 |
|
Description: Rabbit Polyclonal TNF Receptor 1 Antibody. Validated in IP, IHC, WB and tested in Human, Mouse, Rat. |
 Anti-CHST8/Galnac4St 1 Antibody |
|
A10989-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal CHST8/Galnac4St 1 Antibody. Validated in WB and tested in Human, Mouse, Rat. |
 Anti-Pyrophosphatase 1/PPA1 Antibody |
|
A07485-1 |
BosterBio |
100ug/vial |
EUR 334 |
 Anti-TCP-1 eta Antibody |
|
A08169-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for TCP-1 eta Antibody (CCT7) detection. Tested with WB in Human, Mouse, Rat. |
 Anti-Relaxin 1/RLN1 Antibody |
|
A08367-1 |
BosterBio |
100ug/vial |
EUR 334 |
 Anti-TCP-1 zeta Antibody |
|
A09373-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for TCP-1 zeta Antibody (CCT6A) detection.tested for WB in Human, Mouse, Rat. |
 Anti-Galectin 1/Lgals1 Antibody |
|
A00470-1 |
BosterBio |
100ug/vial |
EUR 334 |
 Anti-LOX-1/OLR1 Antibody |
|
A00760-1 |
BosterBio |
100ug/vial |
EUR 294 |
 Anti-Syndecan-1/SDC1 Antibody |
|
A00991-1 |
BosterBio |
100ug/vial |
EUR 294 |
 Anti-IRAK-1/IRAK1 Antibody |
|
A01021-1 |
BosterBio |
100ug/vial |
EUR 334 |
 Anti-Neurexin 1/NRXN1 Antibody |
|
A01490-1 |
BosterBio |
100ug/vial |
EUR 334 |
 Anti-Hexokinase 1/HK1 Antibody |
|
A01504-1 |
BosterBio |
100ug/vial |
EUR 334 |
 Anti-Synaptotagmin 1/SYT1 Antibody |
|
A02314-1 |
BosterBio |
100ug/vial |
EUR 334 |
 Anti-Syntenin-1 Monoclonal Antibody |
|
A02475-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Mouse Monoclonal Antibody for Syntenin-1 Antibody (SDCBP) detection. Tested with WB in Human, Rat, Dog, Pig. |
 Anti-Dsg1/Desmoglein 1 Antibody |
|
A02655-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for Dsg1 Antibody (DSG1) detection.tested for IHC, WB in Human, Mouse, Rat. |
 Anti-CAF-1 p150 Antibody |
|
A02732-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for CAF-1 p150 Antibody (CHAF1A) detection. Tested with WB in Human, Mouse. |
 Anti-Talin 1/TLN1 Antibody |
|
A02859-1 |
BosterBio |
100ug/vial |
EUR 334 |
 Anti-Islet 1/ISL1 Antibody |
|
A02969-1 |
BosterBio |
100ug/vial |
EUR 334 |
 Anti-Musashi 1/Msi1 Antibody |
|
A05052-1 |
BosterBio |
100ug/vial |
EUR 334 |
, 2mg/mL) CF350 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
|
20245-1 |
Biotium |
50uL |
EUR 149 |
|
Description: Minimum order quantity: 1 unit of 50uL |
, 2mg/mL) CF488A Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
|
20246-1 |
Biotium |
50uL |
EUR 149 |
|
Description: Minimum order quantity: 1 unit of 50uL |
, 2mg/mL) CF555 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
|
20247-1 |
Biotium |
50uL |
EUR 149 |
|
Description: Minimum order quantity: 1 unit of 50uL |
, 2mg/mL) CF568 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
|
20248-1 |
Biotium |
50uL |
EUR 149 |
|
Description: Minimum order quantity: 1 unit of 50uL |
, 2mg/mL) CF594 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
|
20249-1 |
Biotium |
50uL |
EUR 149 |
|
Description: Minimum order quantity: 1 unit of 50uL |
, 2mg/mL) CF633 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
|
20250-1 |
Biotium |
50uL |
EUR 149 |
|
Description: Minimum order quantity: 1 unit of 50uL |
, 2mg/mL) CF640R Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
|
20251-1 |
Biotium |
50uL |
EUR 149 |
|
Description: Minimum order quantity: 1 unit of 50uL |
, 2mg/mL) CF647 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
|
20252-1 |
Biotium |
50uL |
EUR 149 |
|
Description: Minimum order quantity: 1 unit of 50uL |
, 2mg/mL) CF680 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
|
20253-1 |
Biotium |
50uL |
EUR 161 |
|
Description: Minimum order quantity: 1 unit of 50uL |
, 2mg/mL) CF770 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
|
20254-1 |
Biotium |
50uL |
EUR 161 |
|
Description: Minimum order quantity: 1 unit of 50uL |
, 2mg/mL) CF543 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
|
20325-1 |
Biotium |
50uL |
EUR 149 |
|
Description: Minimum order quantity: 1 unit of 50uL |
 Prostate Stem Cell Antigen Antibody |
|
33476-100ul |
SAB |
100ul |
EUR 252 |
Prostate Stem Cell Antigen Antibody |
|
33476-50ul |
SAB |
50ul |
EUR 187 |
Prostate Stem Cell Antigen Antibody |
|
DF8709 |
Affbiotech |
200ul |
EUR 304 |
|
Description: Prostate Stem Cell Antigen Antibody detects endogenous levels of total Prostate Stem Cell Antigen. |
) Hemokinin 1 (mouse) |
|
B5127-1 |
ApexBio |
1 mg |
EUR 321 |
 Anti-BOB-1/OBF-1 Rabbit Monoclonal Antibody, Clone#RM378 |
|
M04431-1 |
BosterBio |
100uL |
EUR 385 |
|
Description: Anti-BOB-1/OBF-1 Rabbit Monoclonal Antibody, Clone#RM378 tested in WB, IHC, reactive to Human |
 AXMIR-1 RNA oligo anti-miRNA-1-3p with Xmotif |
|
AXMIR-1 |
SBI |
10 reactions |
EUR 438 |
|
|
 Anti-Sumo 1 Rabbit Monoclonal Antibody |
|
M00631-1 |
BosterBio |
100ug/vial |
EUR 397 |
|
Description: Rabbit Monoclonal Sumo 1 Antibody. Validated in IP, IF, IHC, WB and tested in Human, Mouse, Rat. |
 Anti-Cytokeratin 1 Rabbit Monoclonal Antibody |
|
M01639-1 |
BosterBio |
100ug/vial |
EUR 397 |
|
Description: Rabbit Monoclonal Cytokeratin 1 Antibody. Validated in WB and tested in Human, Mouse, Rat. |
) Anti-Mitofusin 1 Antibody (monoclonal, 3H3) |
|
M02172-1 |
BosterBio |
100ug/vial |
EUR 294 |
 Antibody) Anti-Phospho-Beclin-1 (Ser295) Antibody |
|
P00327-1 |
BosterBio |
100ul |
EUR 398 |
|
Description: Rabbit Polyclonal Phospho-Beclin-1 (Ser295) Antibody. Validated in WB and tested in Human. |
 Antibody) Anti-Phospho-Raf-1 (Ser642) Antibody |
|
P00446-1 |
BosterBio |
100ul |
EUR 398 |
|
Description: Rabbit Polyclonal Phospho-Raf-1 (Ser642) Antibody. Validated in WB and tested in Human, Rat. |
 Anti-Integrin alpha 1/ITGA1 Antibody |
|
PA1045-1 |
BosterBio |
100ug/vial |
EUR 334 |
/CASP1 Antibody) Anti-Caspase-1(P20)/CASP1 Antibody |
|
PA1440-1 |
BosterBio |
100ug/vial |
EUR 294 |
 Anti-VEGF Receptor 1/FLT1 Antibody |
|
PA1966-1 |
BosterBio |
100ug/vial |
EUR 294 |
 Anti-HIF-1 alpha/HIF1A Antibody |
|
A00013-1 |
BosterBio |
100ug/vial |
EUR 294 |
 Antibody) Anti-Cleaved-Notch 1 (V1754) Antibody |
|
A00033-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for Cleaved-Notch 1 (V1754) Antibody (NOTCH1) detection.tested for WB in Human, Mouse, Rat. |
 Antibody) Anti-HDAC-1 (C-terminus) Antibody |
|
A00256-1 |
BosterBio |
100uL |
EUR 443 |
|
Description: Rabbit Polyclonal HDAC-1 (C-terminus) Antibody. Validated in IF, IHC, WB and tested in Human. |
 Anti-CELSR3/Flamingo Homolog 1 Antibody |
|
A07204-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal CELSR3/Flamingo Homolog 1 Antibody. Validated in IF and tested in Human, Mouse, Rat. |
 Anti-LPCAT2/Acyltransferase Like 1 Antibody |
|
A07471-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal LPCAT2/Acyltransferase Like 1 Antibody. Validated in WB and tested in Human, Mouse, Rat. |
Anti-VEGF Receptor 1/FLT1 Antibody |
|
A00534-1 |
BosterBio |
100ug/vial |
EUR 294 |
 Anti-Liver Carboxylesterase 1/CES1 Antibody |
|
A01741-1 |
BosterBio |
100ug/vial |
EUR 334 |
 Anti-EBP50/NHERF-1/SLC9A3R1 Antibody |
|
A02427-1 |
BosterBio |
100ug/vial |
EUR 334 |
 Anti-IL1R2/Il 1 Rii Antibody |
|
A03106-1 |
BosterBio |
1 ml |
EUR 397 |
|
Description: Rabbit Polyclonal IL1R2/Il 1 Rii Antibody. Validated in IP, WB and tested in Human. |
 Anti-Hyaluronan synthase 1/HAS1 Antibody |
|
A04784-1 |
BosterBio |
100ug/vial |
EUR 334 |
 TINAGL1 Human, Tubulointerstitial Nephritis Antigen Like 1 Human Recombinant Protein, Sf9 |
|
PROTQ9GZM7-1 |
BosterBio |
Regular: 5ug |
EUR 317 |
|
Description: TINAGL1 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 455 amino acids (22-467a.a.) and having a molecular mass of 51.2kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). TINAGL1 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques. |
 Anti-TTF-1 |
|
DB-089-1 |
DB Biotech |
1 ml |
EUR 750 |
|
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
 (Hematopoietic Stem Cell Marker); Clone 3D3 (Concentrate)) Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker); Clone 3D3 (Concentrate) |
|
RA0264-C.1 |
ScyTek Laboratories |
0.1 ml |
EUR 125 |
Cell morphology and viability, cell cycle, apoptosis, proteomes, and transcriptomes had been assessed. We noticed that SACCS had higher growth-promoting capabilities than FBS. Cell proliferation was promoted even when FBS was changed by 50-70% SACCS; there was no important distinction in cell form or viability. There had been solely slight variations in the cell cycle, apoptosis, proteomes, and transcriptomes of the cells grown in presence of SACCS. Therefore, SACCS has the potential to be an efficient, low-cost, and eco-friendly various to FBS in in vitro tradition.
