Blogging

Femtosecond pulsed laser microscopy: a new tool to assess the in vitro delivered dose of carbon nanotubes in cell culture experiments

Femtosecond pulsed laser microscopy: a new tool to assess the in vitro delivered dose of carbon nanotubes in cell culture experiments

In vitro fashions are extensively used in nanotoxicology. In these assays, a cautious documentation of the fraction of nanomaterials that reaches the cells, i.e. the in vitro delivered dose, is a essential factor for the interpretation of the information. The in vitro delivered dose might be measured by quantifying the quantity of materials in contact …

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3D printing novel in vitro cancer cell culture model systems for lung cancer stem cell study

3D printing novel in vitro cancer cell culture model systems for lung cancer stem cell study

Two-dimensional (2D) in vitro cell cultures and laboratory animals have been used historically because the gold-standard preclinical cancer model systems. However, for cancer stem cell (CSC) research, they exhibit notable limitations on simulating native setting, which depreciate their translatability for medical improvement functions. In this study, totally different three-dimensional (3D) printing platforms have been used …

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Influence of Bacterial Culture Medium on Peptidoglycan Binding of Cell Wall Lytic Enzymes

Influence of Bacterial Culture Medium on Peptidoglycan Binding of Cell Wall Lytic Enzymes

The bacteriolysin lysostaphin (Lst) and endolysin PlyPH are potent modular lytic enzymes with exercise in opposition to clinically-relevant Gram-positive Staphylococcus aureus and Bacillus cereus, respectively. Both enzymes possess an N-terminal catalytic area and C-terminal binding area, with the latter conferring important enzyme specificity. Lst and PlyPH present diminished exercise within the presence of bacterial growth-supporting …

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Co-culture of Human Stem Cell Derived Neurons and Oligodendrocyte Progenitor Cells

Co-culture of Human Stem Cell Derived Neurons and Oligodendrocyte Progenitor Cells

Crosstalk between neurons and oligodendrocytes is essential for correct mind functioning. Multiple co-culture strategies have been developed to check oligodendrocyte maturation, myelination or the impact of oligodendrocytes on neurons. However, most of these strategies comprise cells derived from animal fashions. In the present protocol, we co-culture human neurons with human oligodendrocytes. Neurons and oligodendrocyte precursor …

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Quantifying HIV-1-Mediated Gut CD4 + T Cell Deathin the Lamina Propria Aggregate Culture (LPAC) Model

Quantifying HIV-1-Mediated Gut CD4 + T Cell Deathin the Lamina Propria Aggregate Culture (LPAC) Model

Gut CD4 T cells are main targets of HIV-1 and are massively depleted early throughout an infection. To higher perceive the mechanisms governing HIV-1-mediated CD4 T cell loss of life, we developed the physiologically-relevant Lamina Propria Aggregate Culture (LPAC) mannequin. The LPAC mannequin is right for learning CD4 T cell loss of life induced by …

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A multicenter comparison of quantification methods for antisense oligonucleotide-induced DMD exon 51 skipping in Duchenne muscular dystrophy cell cultures.

A multicenter comparison of quantification methods for antisense oligonucleotide-induced DMD exon 51 skipping in Duchenne muscular dystrophy cell cultures.

BACKGROUND Duchenne muscular dystrophy is a deadly illness brought on by lack of dystrophin. Skipping of exons adjoining to out-of-frame deletions has confirmed to revive dystrophin expression in Duchenne sufferers. Exon 51 has been essentially the most studied goal in each preclinical and medical settings and the provision of standardized procedures to quantify exon skipping …

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Maintaining Elastogenicity of Mesenchymal Stem Cell-Derived Smooth Muscle Cells in Two-Dimensional Culture.

Maintaining Elastogenicity of Mesenchymal Stem Cell-Derived Smooth Muscle Cells in Two-Dimensional Culture.

Abdominal aortic aneurysms (AAAs) are localized expansions of the stomach aorta that develop slowly to rupture. AAA progress is pushed by irreversible elastic matrix breakdown in the aorta wall by chronically upregulated matrix metalloproteases (MMPs). Since grownup vascular easy muscle cells (SMCs) poorly regenerate elastic matrix, we beforehand explored utility of bone marrow mesenchymal stem …

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Muscle Atrophy Marker Expression Differs between Rotary Cell Culture System and Animal Studies.

Muscle Atrophy Marker Expression Differs between Rotary Cell Culture System and Animal Studies.

Muscular atrophy, outlined because the lack of muscle tissue, is a critical subject for immobilized sufferers on Earth and for people throughout spaceflight, the place microgravity prevents regular muscle loading. In vitro modeling is a vital step in understanding atrophy mechanisms and testing countermeasures earlier than animal trials. The most perfect atmosphere for modeling should …

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GFP

Intensive searches for novel green fluorescent protein (GFP)-like fluorescent proteins have identified more than 150 distinct genes that, together with its mutants, cover the excitation range from 380 to 600 nanometers (nm) and the emission range from 440 to 650 nm (see table below). Despite spectral diversity, a family of GFP-like proteins possesses common significant …

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