High Glucose Induces Lipid Accumulation via 25-Hydroxycholesterol DNA-CpG Methylation.

This work investigates the relationship between high-glucose (HG) culture, CpG methylation of genes involved in cell signaling pathways, and the regulation of carbohydrate and lipid metabolism in hepatocytes.

The results indicate that HG leads to an increase in nuclear 25-hydroxycholesterol (25HC), which specifically activates DNA methyltransferase-1 (DNMT1), and regulates gene expression involved in intracellular lipid metabolism.

The results show significant increases in 5mCpG levels in at least 2,225 genes involved in 57 signaling pathways. The hypermethylated genes directly involved in carbohydrate and lipid metabolism are of PI3K, cAMP, insulin, insulin secretion, diabetic, and NAFLD signaling pathways.

SKT (KIAA1217) Antibody

abx234542-100ug 100 ug
EUR 509

Mouse SKT shRNA Plasmid

20-abx980555
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Mouse pre-microRNA Expression Construct mir-219-1

MMIR-219-1-PA-1 Bacterial Streak
EUR 684

Mouse pre-microRNA Expression Construct mir-219-2

MMIR-219-2-PA-1 Bacterial Streak
EUR 684

CTLA 4 Recombinant Protein

96-219 0.2 mg
EUR 401
Description: CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.

Mouse Sickle tail protein (SKT) ELISA Kit

abx389166-96tests 96 tests
EUR 911

Mouse Sickle tail protein, Skt ELISA KIT

ELI-29595m 96 Tests
EUR 865

Human Sickle tail protein homolog, SKT ELISA KIT

ELI-18163h 96 Tests
EUR 824

hCRP FRET 96

CH1021-96 1 Kit
EUR 455

hCRP TRF 96

CHTR1021-96 1 Kit
EUR 482

hAlbumin FRET 96

AH1005-96 1 Kit
EUR 455

hAlbumin TRF 96

AHTR1005-96 1 Kit
EUR 482

hInsulin FRET 96

IH1017-96 1 Kit
EUR 455

hInsulin TRF 96

IHTR1017-96 1 Kit
EUR 482

rIgG FRET 96

RI1024-96 1 Kit
EUR 455

mIgG TRF 96

MIGTR1020-96 1 Kit
EUR 482

Skt ELISA Kit| Mouse Sickle tail protein ELISA Kit

EF014796 96 Tests
EUR 689

Influenza HA (210 - 219)

5-01388 4 x 5mg Ask for price

ATF4 (Ab-219) Antibody

CSB-PA123639-
EUR 335
Description: A polyclonal antibody against ATF4 (Ab-219). Recognizes ATF4 (Ab-219) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

ATF4 (Ab-219) Antibody

CSB-PA123639-100ul 100ul
EUR 316
Description: A polyclonal antibody against ATF4 (Ab-219). Recognizes ATF4 (Ab-219) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

Influenza HA (210 219) Peptide

20-abx266173
  • EUR 439.00
  • EUR 718.00
  • EUR 328.00
  • 10 mg
  • 25 mg
  • 5 mg

Polyclonal RAB37 Antibody (aa170-219)

AMM07470G 0.05ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human RAB37 (aa170-219). This antibody is tested and proven to work in the following applications:

ATF-4 (Ab-219) Antibody

33307-100ul 100ul
EUR 252

ATF-4 (Ab-219) Antibody

33307-50ul 50ul
EUR 187

mmu-miR-219-3p Primers

MPM00250 150 ul / 10 uM
EUR 121

mmu-miR-219-5p Primers

MPM00251 150 ul / 10 uM
EUR 121

rno-miR-219-5p Primers

MP-r00147 150 ul / 10 uM
EUR 176

hsa-miR-219-5p Primers

MPH01276 150 ul / 10 uM
EUR 121

HSC70 ELISA Kit

SKT-106-96 1 plate of 96 wells
EUR 717
Description: Sandwich ELISA kit used for quantitate HSC70 concentration in Cell Lysates, Tissue, Serum, Whole Blood samples from Human, Avian

HSP90 alpha ELISA Kit

SKT-107-96 1 plate of 96 wells
EUR 685
Description: Sandwich ELISA kit used for quantitate HSP90 alpha concentration in Cell Lysates, Tissue, Serum, Whole Blood samples from Human, Goat, Avian

HSP70 ELISA Kit (High-Sensitivity)

SKT-108-96 1 plate of 96 wells
EUR 685
Description: Sandwich ELISA kit used for quantitate HSP70 concentration in Serum, Plasma, Cell Lysates, Tissue samples from Human, Monkey, Dog, Rat, Mouse

HSP27 ELISA Kit

SKT-109-96 1 plate of 96 wells
EUR 585
Description: Sandwich ELISA kit used for quantitate HSP27 concentration in Serum, Plasma, Cell Lysates, Tissue samples from Human

HSP60 ELISA Kit

SKT-110-96 1 plate of 96 wells
EUR 585
Description: Sandwich ELISA kit used for quantitate HSP60 concentration in Cell Lysates, Tissue, Serum samples from Human

HO-1 ELISA Kit

SKT-111-96 1 plate of 96 wells
EUR 585
Description: Sandwich ELISA kit used for quantitate HO-1 concentration in Cell Lysates, Tissue, Serum, Cyst fluid samples from Human, Rat

HSP65 ELISA Kit

SKT-113-96 1 plate of 96 wells
EUR 633
Description: Sandwich ELISA kit used for quantitate M bovis/M tuberculosum HSP65 concentration in human samples in Cell Lysates, Tissue, Serum samples from M bovis, M tuberculosum

Alpha B Crystallin ELISA Kit

SKT-123-96 1 plate of 96 wells
EUR 717
Description: Sandwich ELISA kit used for quantitate alpha B crystallin concentration in Cell Lysates, Tissue, Serum samples from Human

HSP70 ELISA Kit (PLANT)

SKT-124-96 1 plate of 96 wells
EUR 717
Description: Sandwich ELISA kit used for quantitate HSP70 concentration in Plant extract, Cell Lysates, Tissue, Serum, Plasma samples from Plant, Human

Nitrotyrosine ELISA Kit

SKT-126-96 1 plate of 96 wells
EUR 460
Description: Competitive ELISA kit used for quantitate Nitrotyrosine in Plasma, Serum, Cell Lysates, Urine samples from all species

Rat GRP78 ELISA Kit

SKT-137-96 1 plate of 96 wells
EUR 632
Description: Sandwich ELISA kit used for quantitate GRP78 concentration in rat samples in Tissue samples from Rat

Dibromo-tyrosine ELISA Kit

SKT-140-96 1 plate of 96 wells
EUR 566
Description: Competitive ELISA kit used to quantify free and protein-bound 3,5-dibromotyrosine in Plasma, Urine samples from all species

Cortisol EIA Kit

SKT-201-96 1 plate of 96 wells
EUR 329
Description: Competitive EIA kit used for quantitative measuring cortisol present in Dried Fecal Samples, Saliva, Urine, Serum, EDTA Plasma, Heparin Plasma, Tissue Culture Media samples from all species

Glutathione Detection Kit

SKT-202-96 1 plate of 96 wells
EUR 428
Description: Direct Fluorometric detection assay to measure the total GSH content in Whole Blood, Serum, EDTA Plasma, Heparin Plasma, Erythrocytes, Urine, Cell Lysates, Tissue samples from all species

Glutathione S-Transferase Activity Kit

SKT-203-96 1 plate of 96 wells
EUR 428
Description: Enzyme Activity Fluorometric assay used for quantitative measuring the activity of glutathione S-transferase in Urine, Serum, EDTA Plasma, Heparin Plasma, Cell Lysates samples from Human, Mollusk, Fish

Glutathione Reductase Activity Kit

SKT-204-96 1 plate of 96 wells
EUR 428
Description: Enzyme Activity Fluorometric assay used for measuring glutathione reductase activity by the amount of GSH generated from the reduction of GSSG, in Serum, EDTA Plasma, Heparin Plasma, Erythrocytes, Cell Lysates samples from Human

Corticosterone EIA Kit

SKT-205-96 1 plate of 96 wells
EUR 355
Description: Sandwich EIA kit used to measure the corticosterone present in Serum, EDTA Plasma, Heparin Plasma, Urine, Tissue Culture Media, Dried Fecal Samples samples from all species

Cortisone CLIA Kit

SKT-206-96 1 plate of 96 wells
EUR 467
Description: Sandwich CLIA kit used for quantitative measuring the cortisone present in Dried Fecal Samples, Serum, Saliva, Urine, Plasma, Tissue Culture Media samples from all species

Prostaglandin E2 EIA Kit

SKT-207-96 1 plate of 96 wells
EUR 527
Description: Sandwich EIA kit used for quantitative measuring PGE2 present in Saliva, Urine, Serum, EDTA Plasma, Heparin Plasma, Tissue Culture Media samples from all species

Cyclic AMP CLIA Kit (High-Sensitivity)

SKT-208-96 1 plate of 96 wells
EUR 494
Description: Direct CLIA kit used for quantitative measuring cAMP present in Cell Lysates, Tissue, Saliva, Urine, EDTA Plasma, Heparin Plasma, Tissue Culture Media samples from all species

Cyclic AMP EIA Kit

SKT-209-96 1 plate of 96 wells
EUR 454
Description: Direct EIA kit used for quantitative measuring cAMP present in Cell Lysates, Tissue, Saliva, Urine, EDTA Plasma, Heparin Plasma, Tissue Culture Media samples from all species

Cyclic GMP CLIA Kit (High-Sensitivity)

SKT-210-96 1 plate of 96 wells
EUR 520
Description: Direct CLIA kit used to measure cGMP present in Cell Lysates, Tissue, Saliva, Urine, EDTA Plasma, Tissue Culture Media samples from all species

Cyclic GMP EIA Kit

SKT-211-96 1 plate of 96 wells
EUR 474
Description: Direct EIA kit used for quantitative measuring cGMP present in Cell Lysates, Tissue, Saliva, Urine, EDTA Plasma, Tissue Culture Media samples from all species

Nitric Oxide Detection Kit

SKT-212-96 2 plates of 96 wells
EUR 342
Description: Indirect Colorimetric assay used for quantitative measuring Nitrate and Nitrite present in a variety of samples in Serum, Plasma, Urine, Saliva, Water, Buffer, Cell Lysates, Tissue Culture Media samples from all species

Retinol Binding Protein Urinary EIA Kit

SKT-218-96 1 plate of 96 wells
EUR 527
Description: Sandwich EIA kit used to measure RBP levels in urine samples in Urine samples from Human, Rat, Dog, Monkey

HSP70 ELISA Kit

SKT-105-96 1 plate of 96 wells
EUR 581
Description: Sandwich ELISA kit used for quantitate HSP70 concentration in Cell Lysates, Tissue, Serum, Urine samples from Human, Goat, Dog

Tissue Culture Plate, 96 Well

TCP20-96 1 UNIT
EUR 53.48

Aligned Nanofibers 96 Well Plate

3D00012-96 700 nm-PCLs
EUR 110

Bridge-It cAMP-PDE 96

PD1016-96 1 Kit
EUR 455

Recombinant Human CD59 Protein-10His tag

CTP-219 100ug Ask for price
Description: A 10.3 kDa recombinant human CD59 protein with a C-terminal 10-His tag expressed in the mouse myeloma cell line, NS0.

Recombinant P. falciparum MSP-1 [His]

DAGA-219 1 mg
EUR 441

pSMPUW-IRES-Blasticidin Lentiviral Expression Vector

VPK-219 10 µg
EUR 624
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

XMIRXpress lentivector miRNA-219a-5p with Xmotif

XMIRXP-219 10 ug
EUR 677

IL-8 / CXCL8 Recombinant Protein

11-219 0.1 mg
EUR 595.25
Description: Interleukin 8 (IL8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells. There are many receptors on the surface membrane capable of binding IL-8, the most frequently studied types are the G protein-coupled serpentine receptors CXCR1 and CXCR2. Expression and affinity for IL-8 differs between the two receptors (CXCR1 > CXCR2). IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.

SARS Coronavirus spike (HSZ-Cc) Recombinant Protein

20-219 0.1 mg
EUR 605.75
Description: SARS Coronavirus spike (HSZ-Cc) Recombinant Protein

Annexin V ATTO 488 Recombinant Protein

90-219 100 tests
EUR 364.25
Description: Annexin V is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Annexin V competes for phosphatidylserine binding sites (e. g. platelets) with prothrombin or coagulation factors and inhibis the activity of phospholipase A1. Antibodies directed against annexin V are found in patients with a disease called the antiphospholipid syndrome(APS), a thrombophilic disease associated with autoantibodies against phospholipid compounds. Annexin V is used as a probe in the "annexin V affinity assay" to detect cells that have expressed phosphatidylserine on the cell surface, a feature found in apoptosis and other forms of cell death.

Neurocalcin-delta Recombinant Protein

91-219 0.05 mg
EUR 710.75
Description: Neurocalcin-delta (NCALD) is a neuronal calcium-binding protein that belongs to the neuronal calcium sensor (NCS) family. It expressed in mammalian brains. NCALD contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein possesses a Ca2+ /myristoyl switch. It is cytosolic at resting calcium levels. However, elevated intracellular calcium levels induce a conformational change which exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. NCALD protein is thought to be a regulator of G protein-coupled receptor signal transduction.

IL-1 beta Recombinant Protein

92-219 0.05 mg
EUR 542.75
Description: IL1B belongs to the IL-1 family. Interleukin 1 (IL-1) is a family of polypeptide cytokines consisting of two agonists, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2) encoded by two distinct genes and perform identical biological functions. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response. It is identified as endogenous pyrogens, and is reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Pig Lymphocyte Antigen 96 ELISA kit

E07L0307-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Porcine Lymphocyte Antigen 96 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Lymphocyte Antigen 96 ELISA kit

E08L0307-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Canine Lymphocyte Antigen 96 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Lymphocyte Antigen 96 ELISA kit

E04L0307-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rabbit Lymphocyte Antigen 96 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Lymphocyte Antigen 96 ELISA kit

E06L0307-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Goat Lymphocyte Antigen 96 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Lymphocyte Antigen 96 ELISA kit

E03L0307-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Mouse Lymphocyte Antigen 96 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Lymphocyte Antigen 96 ELISA kit

E02L0307-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Lymphocyte Antigen 96 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Lymphocyte Antigen 96 ELISA kit

E01L0307-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Human Lymphocyte Antigen 96 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Lymphocyte Antigen 96 ELISA kit

E09L0307-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Monkey Lymphocyte Antigen 96 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Ring Finger Protein 219 (RNF219) Antibody

20-abx006113
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Ring Finger Protein 219 (RNF219) Antibody

abx029648-400ul 400 ul
EUR 523

Ring Finger Protein 219 (RNF219) Antibody

abx029648-80l 80 µl
EUR 286

Zinc Finger Protein 219 (ZNF219) Antibody

abx031235-400ul 400 ul
EUR 523

Zinc Finger Protein 219 (ZNF219) Antibody

abx031235-80l 80 µl
EUR 286

Zinc Finger Protein 219 (ZNF219) Antibody

abx031236-400ul 400 ul
EUR 523

Zinc Finger Protein 219 (ZNF219) Antibody

abx031236-80l 80 µl
EUR 286

Ring Finger Protein 219 (RNF219) Antibody

20-abx338409
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Ring Finger Protein 219 (RNF219) Antibody

abx237352-100ug 100 ug
EUR 551

hsa-miR-219-5p miRNA Inhibitor

MIH01575 2 x 5.0 nmol
EUR 176

mmu-miR-219-3p miRNA Inhibitor

MIM01527 2 x 5.0 nmol
EUR 176

mmu-miR-219-5p miRNA Inhibitor

MIM01528 2 x 5.0 nmol
EUR 176

rno??miR-219-5p miRNA Inhibitor

MIR01323 2 x 5.0 nmol
EUR 176

hsa-miR-219-5p miRNA Antagomir

MNH01575 2 x 5.0 nmol
EUR 329

mmu-miR-219-3p miRNA Antagomir

MNM01527 2 x 5.0 nmol
EUR 329

mmu-miR-219-5p miRNA Antagomir

MNM01528 2 x 5.0 nmol
EUR 329

rno??miR-219-5p miRNA Antagomir

MNR01323 2 x 5.0 nmol
EUR 329

hsa-miR-219-1-3p Primers

MPH01274 150 ul / 10 uM
EUR 121

hsa-miR-219-2-3p Primers

MPH01275 150 ul / 10 uM
EUR 121

96 WELL CLEAR PCR MICROPLATE

PCR-96-C 10/pk
EUR 145
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

Guinea pig Lymphocyte Antigen 96 ELISA kit

E05L0307-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Guinea pig Lymphocyte Antigen 96 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

96 WELL PCR SEGMENTED PLATE

PCR-96-SG-C 10/pk
EUR 145
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

The studies indicate a close relationship between the increase in nuclear 25HC levels and activation of DNMT1, which may regulate lipid metabolism via DNA CpG methylation. Our results indicate an epigenetic regulation of hepatic cell metabolism that has relevance to some common diseases such as non-alcoholic fatty liver disease and metabolic syndrome.